Department of Plant Protection, Faculty of Agriculture, Assiut University, 71526 Assiut, Egypt.
Department of Zoology, Faculty of Science, Sohag University, 8562 Sohag, Egypt.
Environ Toxicol Pharmacol. 2023 Aug;101:104213. doi: 10.1016/j.etap.2023.104213. Epub 2023 Jul 7.
Acute toxicity experiments were conducted to determine the lethal concentration 50 (LC50) of the Up Grade®46% SL for Oreochromis niloticus. Our results showed that the 96-h LC50 value of UPGR for O. niloticus was 29.16 mg L-1. To study hemato-biochemical effects, fish were exposed for 15 days to individual UPGR at 2.916 mg L-1, individual polyethylene microplastics (PE-MPs) at 10 mg L-1, and to their combinations UPGR+PE-MPs. UPGR exposure induced significant decrease in account of red blood cells (RBCs) and white blood cells (WBCs), platelets, monocytes, neutrophils, eosinophils, and the concentrations of hemoglobin (Hb), hematocrit (Hct), and mean corpuscular hemoglobin concentration (MCHC) than other treatments, compared to the control group. Sub-acute UPGR exposure significantly increase lymphocytes, mean corpuscular volume (MCV), and mean corpuscular hemoglobin (MCH), compared to the control group. In conclusion, UPGR and PE-MPs displayed antagonistic toxic effects due to maybe the sorption of UPGR onto PE-MPs.
急性毒性实验用于确定 Up Grade®46% SL 对奥利亚罗非鱼的半数致死浓度(LC50)。我们的结果表明,UPGR 对奥利亚罗非鱼的 96 小时 LC50 值为 29.16mg/L。为了研究血液生化效应,将鱼暴露于 2.916mg/L 的单独 UPGR、10mg/L 的单独聚乙烯微塑料(PE-MPs)及其组合 UPGR+PE-MPs 中 15 天。与其他处理组相比,UPGR 暴露会导致红细胞(RBCs)和白细胞(WBCs)、血小板、单核细胞、嗜中性粒细胞、嗜酸性粒细胞以及血红蛋白(Hb)、血细胞比容(Hct)和平均红细胞血红蛋白浓度(MCHC)的浓度显著降低,与对照组相比。与对照组相比,亚急性 UPGR 暴露会显著增加淋巴细胞、平均红细胞体积(MCV)和平均红细胞血红蛋白(MCH)。总之,由于 UPGR 可能被吸附到 PE-MPs 上,因此 UPGR 和 PE-MPs 表现出拮抗的毒性效应。