三种新型离子交换树脂与 DEAE-纤维素对伊氏锥虫的分离纯化比较研究

Comparative study of three novel ion exchange resins with DEAE-cellulose for the purification of Trypanosoma evansi.

机构信息

Laboratório de Hemoparasitas e Vetores, Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC). Av. Luís de Camões, 2090, Conta Dinheiro, Lages, SC, 88520-000, Brazil.

A. C. Camargo Cancer Center, R. Prof. Antônio Prudente, 211, Liberdade, São Paulo, SP, 01509-001, Brazil.

出版信息

Anal Biochem. 2023 Sep 1;676:115226. doi: 10.1016/j.ab.2023.115226. Epub 2023 Jul 6.

DOI:10.1016/j.ab.2023.115226

PMID:37422062

Abstract

Ion exchange chromatography is a method that uses the different surface charges of trypanosomes and blood cells to separate them. This makes it possible to use molecular and immunological methods to diagnose or study these protozoans. DEAE-cellulose resin is commonly used to perform this method. The goal of this study was to compare three novel chromatographic resins designated as PURIFICA™ (Y-C2N®, Y-HONOH®, and Y-CNC3®). The resins were evaluated based on their ability to isolate the parasite, purification time, examination of parasite viability and morphology, and trypanosome recovery potential after passing through the columns. In terms of the evaluated parameters, there was no significant difference between DEAE-cellulose and the three tested resins in most experiments. However, PURIFICA™ (Y-C2N®, Y-HONOH®, and Y-CNC3®) resins are less expensive and easier to prepare than DEAE-Cellulose, making them an alternative for the purification of Trypanosoma evansi.

摘要

离子交换层析是一种利用锥虫和血细胞表面电荷不同来分离它们的方法。这使得使用分子和免疫学方法诊断或研究这些原生动物成为可能。常用 DEAE-纤维素树脂来进行这种方法。本研究的目的是比较三种新型的色谱树脂，分别命名为 PURIFICA™（Y-C2N®, Y-HONOH®, 和 Y-CNC3®）。根据它们分离寄生虫的能力、纯化时间、寄生虫活力和形态检查以及通过柱子后锥虫回收潜力来评估这些树脂。在所评估的参数方面，在大多数实验中，DEAE-纤维素和三种测试的树脂之间没有显著差异。然而，PURIFICA™（Y-C2N®, Y-HONOH®, 和 Y-CNC3®）树脂比 DEAE-纤维素更便宜且更容易制备，因此是纯化伊氏锥虫的替代方法。