Universidade do Estado de Santa Catarina, Laboratório de Bioquímica de Hemoparasitas e Vetores - LABHEV, Avenida Luiz de Camões, n° 2090, Bairro Conta Dinheiro Lages 88520-000, SC, Brazil.
Res Vet Sci. 2011 Apr;90(2):257-9. doi: 10.1016/j.rvsc.2010.05.021. Epub 2010 Jun 9.
Cryopreservation is a method of keeping parasites alive in a laboratory. However, this technique may also damage the parasite. Alternatively, parasites may be maintained by in vitro culture. Unfortunately, for Trypanosoma evansi no effective medium that is able to maintain the parasite for more than 4 months has been described. In this study, we examined the effect of purifying trypomastigote through DEAE-cellulose chromatography before and after cryopreservation, by analyzing the pre-patent period, longevity, parasitemia, and count of viable parasites. Our results showed a three-times increase in the concentration of viable trypomastigote in DEAE-purified cryopreserved parasites as compared to non-DEAE-purified cryopreserved parasites. This indicates that DEAE-cellulose chromatography followed by cryopreservation is an effective method for the storage and preservation of T. evansi, with the advantage that the stocked parasites will be ready to use in molecular biology procedures.
冷冻保存是一种在实验室中保持寄生虫存活的方法。然而，这种技术也可能会损害寄生虫。或者，可以通过体外培养来维持寄生虫。不幸的是，对于伊氏锥虫，尚未有描述能够将寄生虫维持超过 4 个月的有效培养基。在这项研究中，我们通过分析潜伏前期、寿命、寄生虫血症和活寄生虫计数，检查了冷冻保存前后通过 DEAE-纤维素层析法纯化锥虫的效果。我们的结果表明，与未经过 DEAE 纯化的冷冻保存寄生虫相比，DEAE 纯化冷冻保存寄生虫中的活锥虫浓度增加了三倍。这表明，DEAE-纤维素层析法随后进行冷冻保存是一种有效的伊氏锥虫储存和保存方法，其优点是储存的寄生虫将随时可用于分子生物学程序。
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