基于 Cas-12a 的三聚体 G-四链体顺式切割活性的 ASFV 可视化等温扩增检测。
Visual isothermal amplification detection of ASFV based on trimeric G-quadruplex cis-cleavage activity of Cas-12a.
机构信息
School of Life Sciences, Shanghai University, Shanghai, 200444, China.
School of Life Sciences, Shanghai University, Shanghai, 200444, China.
出版信息
Anal Biochem. 2023 Sep 1;676:115235. doi: 10.1016/j.ab.2023.115235. Epub 2023 Jul 6.
African swine fever virus (ASFV) is a kind of DNA virus and can infect both domestic pigs and wild boars with fatality up to 100%. The contaminated meat products mainly led to the worldwide transmission of ASFV. The outbreak of ASF greatly affects the supply stability of meat products as well as the development of the global pig industry. In this study, a visual isothermal amplification detection assay for ASFV based on trimeric G-quadruplex cis-cleavage activity of Cas12a was developed. The introduction of Cas12a could discriminate the specific amplification from the non-specific amplification and improve the sensitivity. The detection limit was as low as 0.23 copies/μL. This assay had good potential in the detection of ASFV and would be helpful for the stability of meat production and supply.
非洲猪瘟病毒（ASFV）是一种 DNA 病毒，可感染家猪和野猪，死亡率高达 100%。受污染的肉类产品主要导致了 ASFV 的全球传播。ASF 的爆发极大地影响了肉类产品的供应稳定性以及全球养猪业的发展。本研究基于 Cas12a 的三聚体 G-四链体顺式切割活性，开发了一种用于检测 ASFV 的可视化等温扩增检测方法。Cas12a 的引入可以区分特异性扩增和非特异性扩增，提高了灵敏度。检测限低至 0.23 拷贝/μL。该方法在 ASFV 的检测中具有良好的应用前景，将有助于保障肉类生产和供应的稳定性。
Zotero 插件