递增整体心肌缺血时间与梗死面积：环孢素 A 在循环死亡大鼠心脏供体中的作用。
Infarct Size With Incremental Global Myocardial Ischemia Times: Cyclosporine A in Donation After Circulatory Death Rat Hearts.
机构信息
Division of Cardio-Thoracic Surgery, Virginia Commonwealth University, Richmond, Virginia; Department of Surgery, McGuire Veterans Administration Medical Center and Pauley Heart Center, Virginia Commonwealth University, Richmond, Virginia.
Division of Cardio-Thoracic Surgery, Virginia Commonwealth University, Richmond, Virginia.
出版信息
Transplant Proc. 2023 Sep;55(7):1495-1503. doi: 10.1016/j.transproceed.2023.03.088. Epub 2023 Jul 7.
BACKGROUND
We quantified the myocardial infarct size with varying global ischemia durations and studied the benefits of Cyclosporine A (CyA) in reducing cardiac injury in ex vivo and transplanted rat hearts.
METHODS
Infarct size was measured after 15, 20, 25, 30, and 35 minutes of in vivo global ischemia (n = 34) and compared with control beating-heart donor (CBD) hearts (n = 10). For heart function assessment, donation after circulatory death (DCD) rat hearts (n = 20) were procured after 25 minutes of in vivo ischemia and reanimated ex vivo for 90 minutes. Half of the DCD hearts received CyA (0.5 mM) at reanimation. The CBD hearts (n = 10) served as controls. A separate group of CBD and DCD (with or without CyA treatment) hearts underwent heterotopic heart transplantation; heart function was measured at 48 hours.
RESULTS
Infarct size was 25% with 25 minutes of ischemia and increased significantly with 30 and 35 minutes to 32% and 41%, respectively. CyA treatment decreased infarct size in DCD hearts (15% vs 25%). Heart function in the transplanted DCD hearts was significantly better with CyA treatment and was comparable to CBD hearts.
CONCLUSIONS
CyA administered at reperfusion limited infarct size in DCD hearts and improved their function in transplanted hearts.
背景
我们量化了不同整体缺血持续时间的心肌梗死面积，并研究了环孢素 A（CyA）在减少体外和移植大鼠心脏损伤方面的益处。
方法
在体内整体缺血 15、20、25、30 和 35 分钟后测量梗死面积，并与对照搏动心脏供体（CBD）心脏（n=10）进行比较。为了评估心脏功能，在体内缺血 25 分钟后获取捐赠后循环死亡（DCD）大鼠心脏（n=20），并在体外再灌注 90 分钟。一半的 DCD 心脏在再灌注时接受 CyA（0.5 mM）治疗。CBD 心脏（n=10）作为对照。一组单独的 CBD 和 DCD（有或没有 CyA 治疗）心脏进行异位心脏移植；在 48 小时测量心脏功能。
结果
缺血 25 分钟的梗死面积为 25%，随着缺血 30 和 35 分钟分别增加到 32%和 41%。CyA 治疗可减少 DCD 心脏的梗死面积（15%对 25%）。用 CyA 治疗的移植 DCD 心脏的心脏功能明显更好，与 CBD 心脏相当。
结论
在再灌注时给予 CyA 可限制 DCD 心脏的梗死面积，并改善其在移植心脏中的功能。
Zotero 插件