Institute for Biochemistry, Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Cologne, Zuelpicher Str. 47a, 50674 Cologne, Germany.
Institute for Biochemistry, Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Cologne, Zuelpicher Str. 47a, 50674 Cologne, Germany.
Cell Signal. 2023 Sep;109:110796. doi: 10.1016/j.cellsig.2023.110796. Epub 2023 Jul 7.
Personal medicine aims to provide tailor-made diagnostics and treatments and has been emerged as a promising but challenging strategy during the last years. This includes the active delivery and localization of a therapeutic compound to a targeted site of action within a cell. An example being targeting the interference of a distinct protein-protein interaction (PPI) within the cell nucleus, mitochondria or other subcellular location. Therefore, not only the cell membrane has to be overcome but also the final intracellular destination has to be reached. One approach which fulfills both requirements is to use short peptide sequences that are able to translocate into cells as targeting and delivery vehicles. In fact, recent progress in this field demonstrates how these tools can modulate the pharmacological parameters of a drug without compromising its biological activity. Beside classical targets that are addressed by various small molecule drugs such as receptors, enzymes, or ion channels, PPIs have received increasing attention as potential therapeutic targets. Within this review, we will provide a recent update on cell-permeable peptides targeting subcellular destinations. We include chimeric peptide probes that combine cell-penetrating peptides (CPPs) and a targeting sequence, as well peptides having intrinsic cell-permeability and which are often used to target PPIs.
个性化医疗旨在提供定制化的诊断和治疗方法，近年来已成为一种有前途但具有挑战性的策略。这包括将治疗性化合物主动递送至细胞内的靶向作用部位并定位于此。例如，靶向干扰细胞核、线粒体或其他亚细胞位置内的特定蛋白质-蛋白质相互作用 (PPI)。因此，不仅要克服细胞膜，还要到达最终的细胞内目的地。一种满足这两个要求的方法是使用能够作为靶向和递药载体进入细胞的短肽序列。事实上，该领域的最新进展表明，这些工具如何在不影响药物生物学活性的情况下调节其药理参数。除了受体、酶或离子通道等各种小分子药物所针对的经典靶点外，PPI 作为潜在的治疗靶点受到越来越多的关注。在本综述中，我们将提供针对亚细胞靶标的细胞穿透肽的最新进展。我们包括结合了细胞穿透肽 (CPP) 和靶向序列的嵌合肽探针，以及具有内在细胞穿透性且常用于靶向 PPI 的肽。
