肾成纤维细胞的增殖、培养和特性鉴定。

Propagation, Culture, and Characterization of Renal Fibroblasts.

机构信息

Department of Nephrology, The Royal Melbourne Hospital, Parkville, Melbourne, VIC, Australia.

Department of Medicine, University of Melbourne, Parkville, Melbourne, VIC, Australia.

出版信息

Methods Mol Biol. 2023;2664:13-29. doi: 10.1007/978-1-0716-3179-9_2.

DOI:10.1007/978-1-0716-3179-9_2

PMID:37423979

Abstract

The renal fibroblast, and phenotypically related myofibroblast, are universally present in all forms of progressive kidney disease. The in vitro study of the fibroblast, its behaviour, and factors affecting its activity is therefore key to understanding both its role and significance. In this protocol, we describe a reproducible method for selective propagation and culture of primary renal fibroblasts from kidney cortex. Techniques for their isolation, subculture, characterization, and cryogenic storage and retrieval are described in detail.

摘要

肾脏成纤维细胞和表型相关的肌成纤维细胞普遍存在于所有形式的进行性肾脏疾病中。因此，体外研究成纤维细胞及其行为以及影响其活性的因素对于理解其作用和意义至关重要。在本方案中，我们描述了一种从肾脏皮质中选择性增殖和培养原代肾成纤维细胞的可重复方法。详细描述了它们的分离、传代、鉴定以及低温储存和检索的技术。