Koo G C, Peppard J R, Lattime E C
Cell Immunol. 1986 Mar;98(1):172-80. doi: 10.1016/0008-8749(86)90277-7.
Bone marrow (BM) harbors precursors (Pre-NK) to NK cells. Recently, we devised an in vitro culture system that induces differentiation of the presumptive BM Pre-NK cells into cytotoxic cells to YAC in the presence of rat concanavalin A (Con A) conditioned medium. We have now analyzed the antigenic phenotype of the effector cells, precursor cells, and the target specificity of these cytotoxic cells. The cytotoxic cells had antigenic profiles similar to endogenous NK cells with the exception of Lyt-2 antigen. They are strongly positive for Qa-5, Thy-1, and partially positive for NK-1, Ly-5, Ly-6, Ly-10, and AsGm-1 and Lyt-2 antigens. The Pre-NK or accessory cells are positive for Qa-5, Ly-10, and Ly-20 and partially positive for NK-1, Thy-1, and AsGm-1 antigens. These Qa-5+ NK cells do not exhibit cytotoxic activity to WEHI or P815. They could also be generated from BM of nude mice as well as beige mice. We concluded from these studies that rat Con A-conditioned medium contained factors that could differentiate Pre-NK cells to mature NK cells and that these cells are heterogeneous. This in vitro culture system is useful in delineating the ontogeny of NK cells.
骨髓（BM）中含有自然杀伤细胞（NK细胞）的前体细胞（Pre-NK）。最近，我们设计了一种体外培养系统，该系统能在大鼠伴刀豆球蛋白A（Con A）条件培养基存在的情况下，诱导假定的骨髓Pre-NK细胞分化为对YAC具有细胞毒性的细胞。我们现在分析了效应细胞、前体细胞的抗原表型以及这些细胞毒性细胞的靶标特异性。除了Lyt-2抗原外，这些细胞毒性细胞的抗原谱与内源性NK细胞相似。它们对Qa-5、Thy-1呈强阳性，对NK-1、Ly-5、Ly-6、Ly-10和AsGm-1以及Lyt-2抗原呈部分阳性。Pre-NK或辅助细胞对Qa-5、Ly-10和Ly-20呈阳性，对NK-1、Thy-1和AsGm-1抗原呈部分阳性。这些Qa-5+NK细胞对WEHI或P815不表现出细胞毒性活性。它们也可以从小鼠和米色小鼠的骨髓中产生。我们从这些研究中得出结论，大鼠Con A条件培养基中含有能将Pre-NK细胞分化为成熟NK细胞的因子，并且这些细胞是异质性的。这种体外培养系统有助于描绘NK细胞的个体发生。
