Luo Qianyi, Hajrasouliha Amir R, Bhatwadekar Ashay D
Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute, Indiana University, Indianapolis, IN, United States.
Front Ophthalmol (Lausanne). 2023;3. doi: 10.3389/fopht.2023.1116838. Epub 2023 Feb 27.
Diabetic retinopathy (DR) is a leading cause of vision impairment. The proliferative form of DR (PDR) involves fibrovascular membrane (FVM) formation at the vitreoretinal interface. MicroRNAs (miRNAs) are a class of non-coding RNA molecules that play an important role in gene regulation; a single miRNA could regulate multiple genes. We previously reported that miR-92a, a suppressor of integrins α and α, was downregulated in DR. Considering the integrin's role in FVM pathology and the potential involvement of miR-92a in DR, we asked a question whether miR-92a could play a critical role in FVM pathology. We collected the FVM and epiretinal membranes of individuals with PDR and macular pucker (control) undergoing pars plana vitrectomy. The frozen sections of membranes were stained for α and αβ integrins. The miR-92a levels were assessed using real-time quantitative PCR. The FVMs of individuals with PDR stained brighter for integrin subunits α and αβ compared to the epiretinal membranes of subjects with macular pucker. miR-92a levels were decreased in FVM subjects. In conclusion, our studies demonstrate that miR-92a decrease is associated with an increase in integrins α and αβ, thus contributing to the inflammatory milieu in PDR.
糖尿病性视网膜病变（DR）是视力损害的主要原因。增殖型糖尿病性视网膜病变（PDR）涉及玻璃体视网膜界面处纤维血管膜（FVM）的形成。微小RNA（miRNA）是一类在基因调控中起重要作用的非编码RNA分子；单个miRNA可调控多个基因。我们之前报道过，作为整合素α和α的抑制剂，miR-92a在DR中表达下调。考虑到整合素在FVM病理中的作用以及miR-92a在DR中的潜在参与，我们提出一个问题，即miR-92a是否可能在FVM病理中起关键作用。我们收集了接受玻璃体切割术的PDR和黄斑皱襞患者（对照）的FVM和视网膜前膜。对膜的冰冻切片进行整合素α和αβ染色。使用实时定量PCR评估miR-92a水平。与黄斑皱襞患者的视网膜前膜相比，PDR患者的FVM中整合素亚基α和αβ染色更亮。FVM患者的miR-92a水平降低。总之，我们的研究表明，miR-92a的降低与整合素α和αβ的增加有关，从而导致PDR中的炎症环境。
