Akhtar Nahid, Mannan M Amin-Ul, Pandey Deeksha, Sarkar Amon, Sharma Himanshi, Kumar Manish, Ghosh Anup
Department of Molecular Biology and Genetic Engineering, School of Bioengineering and Biosciences, Lovely Professional University, Jalandhar-Delhi, India.
Division of Infectious Disease, The Lundquist Institute for Biomedical Innovation at Harbor, University of California Los Angeles (UCLA) Medical Center, Los Angeles, CA, USA.
BioTechnologia (Pozn). 2023 Jun 26;104(2):105-119. doi: 10.5114/bta.2023.127202. eCollection 2023.
is a major public health concern due to its high transmission and mortality rates, as well as the emergence of pan-resistant strains. This study aimed to identify an antifungal compound from , an ethnomedicinal plant, that can inhibit the growth of . Methanol and ethyl acetate extracts of the plant were obtained, and high-performance thin-layer chromatography (HPTLC) analysis was conducted to identify the major compounds in the extracts. The major compound detected by HPTLC was subjected to antifungal activity testing, and its antifungal mechanism was determined. The plant extracts inhibited the growth of both and . HPTLC analysis revealed the presence of gallic acid in the leaf extract. Furthermore, the in vitro antifungal assay showed that gallic acid inhibited the growth of different strains. studies indicated that gallic acid can bind to the active sites of carbonic anhydrase (CA) proteins in both and , affecting their catalytic activities. Compounds that target virulent proteins such as CA can aid in the reduction of drug-resistant fungi and the development of novel antifungal compounds with unique modes of action. However, additional and clinical studies are required to conclusively determine gallic acid's antifungal properties. Gallic acid derivatives may be developed in the future to possess more potent antifungal properties and target various pathogenic fungi.
由于其高传播率和死亡率，以及泛耐药菌株的出现，它成为一个主要的公共卫生问题。本研究旨在从一种民族药用植物中鉴定出一种能抑制其生长的抗真菌化合物。获得了该植物的甲醇和乙酸乙酯提取物，并进行了高效薄层色谱（HPTLC）分析以鉴定提取物中的主要化合物。对HPTLC检测到的主要化合物进行抗真菌活性测试，并确定其抗真菌机制。植物提取物抑制了其生长。HPTLC分析表明叶提取物中存在没食子酸。此外，体外抗真菌试验表明没食子酸抑制了不同菌株的生长。研究表明没食子酸可与二者中的碳酸酐酶（CA）蛋白的活性位点结合，影响其催化活性。靶向CA等毒力蛋白的化合物有助于减少耐药真菌，并开发具有独特作用模式的新型抗真菌化合物。然而，需要更多的研究和临床研究来最终确定没食子酸的抗真菌特性。未来可能会开发没食子酸衍生物，使其具有更强的抗真菌特性并靶向各种致病真菌。
