Léotard B, Marchand-Arvier M, Vigneron C
Clin Chem. 1986 Sep;32(9):1779-81.
We describe an ultramicromethod for measurement of terminal deoxynucleotidyl transferase activity. The materials used are as proposed by Hösli (Clin Chem 1977;23:1476-84) for antenatal diagnosis. The technique is based on the methods of Beutler and Kuhl (Am J Clin Pathol 1978;70:733-7) and Yasmineh et al. (Clin Chem 1980; 26:891-5). The procedure makes it possible to reduce the blood sample to 2 mL at the most and to reduce the cost of the measurement very considerably. The technique gives results similar to those given by the conventional techniques.
我们描述了一种用于测量末端脱氧核苷酸转移酶活性的超微量方法。所使用的材料如Hösli（《临床化学》1977年；23卷：1476 - 1484页）所提议的那样，用于产前诊断。该技术基于Beutler和Kuhl（《美国临床病理学杂志》1978年；70卷：733 - 737页）以及Yasmineh等人（《临床化学》1980年；26卷：891 - 895页）的方法。该程序能够将血样最多减少至2 mL，并大幅降低测量成本。该技术给出的结果与传统技术给出的结果相似。
