Gleed J H, Hendy G N, Nussbaum S R, Rosenblatt M, O'Riordan J L
Clin Endocrinol (Oxf). 1986 Apr;24(4):365-73. doi: 10.1111/j.1365-2265.1986.tb01640.x.
A new peptide spanning residues 28-54 of human parathyroid hormone (PTH) was synthesized and used to develop a homologous immunoradiometric assay specific for the mid-region of human PTH. The peptide was coupled to cellulose and used to absorb mid-region antibodies from a goat antiserum against intact human PTH. This assay has been applied to the measurement of circulating PTH in man: in normal subjects the concentration in serum ranged from undetectable (less than 40 pg/ml) to 70 pg/ml, the reference standard being the human PTH 28-54 peptide. In patients with primary hyperparathyroidism concentrations ranged from 120 to 1800 pg/ml. Hormone was not detected in patients with hypoparathyroidism. In normal subjects and in patients with primary hyperparathyroidism the mid-region PTH concentrations were similar to those obtained in an amino-terminal specific assay. By contrast, carboxy-terminal PTH concentrations were markedly higher being 10-fold greater in both groups studied. In patients with primary hyperparathyroidism undergoing parathyroidectomy and in chronic renal failure patients who were infused with calcium, mid-region and amino-terminal PTH disappeared much more rapidly than carboxy-terminal PTH. However, although mid-region PTH was initially cleared as quickly as amino-terminal PTH, it then reached a plateau and remained at a higher level. Thus the mid-region specific assay described here is proving to be of value in the study of the secretion and metabolism of PTH.
合成了一种跨越人甲状旁腺激素（PTH）第28 - 54位氨基酸残基的新肽段，并用于开发一种针对人PTH中段的同源免疫放射分析方法。该肽段与纤维素偶联，用于从抗完整人PTH的山羊抗血清中吸收中段抗体。此分析方法已应用于人体循环中PTH的测量：在正常受试者中，血清浓度范围从检测不到（低于40 pg/ml）到70 pg/ml，参考标准为人PTH 28 - 54肽段。原发性甲状旁腺功能亢进患者的浓度范围为120至1800 pg/ml。甲状旁腺功能减退患者未检测到激素。在正常受试者和原发性甲状旁腺功能亢进患者中，中段PTH浓度与氨基末端特异性分析中获得的浓度相似。相比之下，羧基末端PTH浓度明显更高，在两组研究对象中均高出10倍。在接受甲状旁腺切除术的原发性甲状旁腺功能亢进患者和输注钙的慢性肾衰竭患者中，中段和氨基末端PTH的消失速度比羧基末端PTH快得多。然而，尽管中段PTH最初清除速度与氨基末端PTH一样快，但随后达到平台期并维持在较高水平。因此，这里描述的中段特异性分析方法在PTH分泌和代谢研究中被证明具有价值。
