Konagaya Y, Konagaya M, Takatsuki K, Tomita A, Watanabe S, Noda T, Katsuragi S
Jpn J Med. 1984 Aug;23(3):199-204. doi: 10.2169/internalmedicine1962.23.199.
A homologous radioimmunoassay specific for amino-terminal portion of human parathyroid hormone(PTH) was developed in order to study the clearance of biologically active species of the hormone in the circulation in man. Characterization of the assay system with synthetic analogues of human PTH (1-34) indicated that the carboxyl-terminal region of human PTH(1-34) is an important recognition site. Plasma amino-terminal PTH levels were less than 0.3 ng/ml in all of 35 normal subjects. The levels were elevated above 0.3 ng/ml in 15 of 24 patients with primary hyperparathyroidism. In 5 patients in whom the levels were determined before and after parathyroidectomy, the elevated levels were all normalized within 60 minutes after the removal of adenomata. The disappearance of exogenous human PTH(1-34) was studied after intravenous administration in 17 patients with hypoparathyroidism. A graphical analysis of the data disclosed two major components of the disappearance curve with estimated half-disappearance time of 3 and 28 minutes respectively, suggesting that multiple mechanisms are involved in the clearance of the peptide from the circulation. These results demonstrate usefulness of homologous radioimmunoassay for human PTH(1-34) in diagnosis and management of hyperparathyroidism, as well as in studying the clearance of amino-terminal portion of PTH which is known to represent biological activity.
为了研究人体内循环中具有生物活性的甲状旁腺激素（PTH）的清除情况，开发了一种针对人甲状旁腺激素氨基末端部分的同源放射免疫测定法。用人甲状旁腺激素（1 - 34）的合成类似物对该测定系统进行表征表明，人甲状旁腺激素（1 - 34）的羧基末端区域是一个重要的识别位点。35名正常受试者的血浆氨基末端甲状旁腺激素水平均低于0.3 ng/ml。24例原发性甲状旁腺功能亢进患者中有15例的水平升高至0.3 ng/ml以上。在5例甲状旁腺切除术前和术后测定水平的患者中，切除腺瘤后60分钟内，升高的水平均恢复正常。在17例甲状旁腺功能减退患者静脉注射外源性人甲状旁腺激素（1 - 34）后，研究了其消失情况。对数据的图形分析揭示了消失曲线的两个主要成分，估计半衰期分别为3分钟和28分钟，这表明多种机制参与了该肽从循环中的清除。这些结果证明了人甲状旁腺激素（1 - 34）同源放射免疫测定法在甲状旁腺功能亢进的诊断和管理中以及在研究已知代表生物活性的甲状旁腺激素氨基末端部分的清除方面的有用性。
