涡虫模型系统用于研究宿主-病原体相互作用。
A Planarian Model System to Study Host-Pathogen Interactions.
机构信息
Department of Molecular & Cell Biology, University of California, Merced, CA, USA.
Quantitative and Systems Biology Graduate Program, University of California, Merced, CA, USA.
出版信息
Methods Mol Biol. 2023;2680:231-244. doi: 10.1007/978-1-0716-3275-8_14.
Abstract
This protocol is focused on using the recently established planarian infection model system to study host-pathogen interactions during fungal infection. Here, we describe in detail the infection of the planarian Schmidtea mediterranea with the human fungal pathogen Candida albicans. This simple and reproducible model system allows for rapid visualization of tissue damage throughout different infection timepoints. We note that this model system has been optimized for use with C. albicans, but should also be applicable for use with other pathogens of interest.
摘要
本方案专注于利用最近建立的涡虫感染模型系统来研究真菌感染过程中的宿主-病原体相互作用。在这里，我们详细描述了人类真菌病原体白色念珠菌对涡虫 Schmidtea mediterranea 的感染。这个简单且可重复的模型系统可以快速可视化不同感染时间点的组织损伤。我们注意到，该模型系统已针对白色念珠菌进行了优化，但也应该适用于其他感兴趣的病原体。
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本文引用的文献
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2
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Elife. 2020 Apr 2;9:e52613. doi: 10.7554/eLife.52613.
3
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