IEEE Trans Nanobioscience. 2024 Jan;23(1):63-70. doi: 10.1109/TNB.2023.3289787. Epub 2024 Jan 3.
The current study developed a drug delivery system through the green chemistry-based synthesis of a biologically friendly metal-organic framework (bio-MOF) called Asp-Cu, which included copper ions and the environmentally friendly molecule L(+)-aspartic acid (Asp). For the first time, diclofenac sodium (DS) was loaded onto the synthesized bio-MOF simultaneously. The system's efficiency was then improved by encapsulating it with sodium alginate (SA). FT-IR, SEM, BET, TGA, and XRD analyses confirmed that DS@Cu-ASP was successfully synthesized. DS@Asp-Cu was found to release the total load within 2 h when used with simulated stomach media. This challenge was overcome by coating DS@Cu-ASP with SA (SA@DS@Cu-ASP). SA@DS@Cu-ASP displayed limited drug release at pH 1.2, and a higher percentage of the drug was released at pH 6.8 and 7.4 due to the pH-responsive nature of SA. In vitro cytotoxicity screening showed that SA@DS@Cu-ASP could be an appropriate biocompatible carrier with >90% cell viability. The on-command drug carrier was observed to be more applicable biocompatible with lower toxicity, as well as adequate loading properties and responsiveness, indicating its applicability as a feasible drug carrier with controlled release.
本研究通过绿色化学合成一种名为 Asp-Cu 的生物友好型金属有机骨架（bio-MOF），开发了一种药物传递系统，其中包括铜离子和环保分子 L(+)-天冬氨酸（Asp）。首次将双氯芬酸钠（DS）同时负载到合成的 bio-MOF 上。然后，通过用海藻酸钠（SA）对其进行包封来提高系统的效率。FT-IR、SEM、BET、TGA 和 XRD 分析证实成功合成了 DS@Cu-ASP。发现 DS@Asp-Cu 在使用模拟胃液介质时在 2 小时内释放全部负载。通过用 SA（SA@DS@Cu-ASP）对 DS@Cu-ASP 进行涂层克服了这一挑战。SA@DS@Cu-ASP 在 pH 1.2 时显示出有限的药物释放，而由于 SA 的 pH 响应特性，在 pH 6.8 和 7.4 时释放出更高百分比的药物。体外细胞毒性筛选表明，SA@DS@Cu-ASP 可以作为一种合适的生物相容性载体，细胞存活率>90%。观察到这种按需药物载体具有更好的生物相容性、更低的毒性以及足够的负载性能和响应性，表明其作为具有控制释放的可行药物载体的适用性。
