Department of Integrative Biology, University of California, 142 Weill Hall #3200, Berkeley, CA 94720, United States.
Department of Ecology and Evolutionary Biology, Tulane University, New Orleans, LA 70118, United States.
J Hered. 2023 Nov 15;114(6):698-706. doi: 10.1093/jhered/esad043.
The California Pipevine Swallowtail Butterfly, Battus philenor hirsuta, and its host plant, the California Pipevine or Dutchman's Pipe, Aristolochia californica Torr., are an important California endemic species pair. While this species pair is an ideal system to study co-evolution, genomic resources for both are lacking. Here, we report a new, chromosome-level assembly of B. philenor hirsuta as part of the California Conservation Genomics Project (CCGP). Following the sequencing and assembly strategy of the CCGP, we used Pacific Biosciences HiFi long reads and Hi-C chromatin proximity sequencing technology to produce a de novo assembled genome. Our genome assembly, the first for any species in the genus, contains 109 scaffolds spanning 443 mega base (Mb) pairs, with a contig N50 of 14.6 Mb, a scaffold N50 of 15.2 Mb, and BUSCO complete score of 98.9%. In combination with the forthcoming A. californica reference genome, the B. philenor hirsuta genome will be a powerful tool for documenting landscape genomic diversity and plant-insect co-evolution in a rapidly changing California landscape.
加利福尼亚管凤蝶（Battus philenor hirsuta）及其寄主植物加利福尼亚管花马兜铃（Aristolochia californica Torr.）是加利福尼亚地区特有的重要物种对。尽管这一对物种是研究共同进化的理想系统，但两者的基因组资源都很缺乏。在这里，我们作为加利福尼亚保护基因组计划（CCGP）的一部分，报告了加利福尼亚管凤蝶的一个新的染色体水平基因组组装。根据 CCGP 的测序和组装策略，我们使用了 Pacific Biosciences HiFi 长读长和 Hi-C 染色质接近测序技术来产生从头组装的基因组。我们的基因组组装是该属中第一个基因组，包含 109 个 scaffolds，跨越 443 兆碱基对（Mb），contig N50 为 14.6 Mb，scaffold N50 为 15.2 Mb，BUSCO 完整评分达到 98.9%。结合即将发布的加利福尼亚管花马兜铃参考基因组，加利福尼亚管凤蝶的基因组将成为记录加利福尼亚快速变化景观中的景观基因组多样性和植物-昆虫共同进化的有力工具。
