Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China.
Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China; Guangzhou Key Laboratory of Drug Research for Emerging Virus Prevention and Treatment, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China.
Antiviral Res. 2023 Aug;216:105666. doi: 10.1016/j.antiviral.2023.105666. Epub 2023 Jul 8.
Zika virus (ZIKV) has garnered global attention due to its association with severe congenital defects including microcephaly. However, there are no licensed vaccines or drugs against ZIKV infection. Pregnant women have the greatest need for treatment, making drug safety crucial. Alpha-linolenic acid (ALA), a polyunsaturated ω-3 fatty acid, has been used as a health-care product and dietary supplement due to its potential medicinal properties. Here, we demonstrated that ALA inhibits ZIKV infection in cells without loss of cell viability. Time-of-addition assay revealed that ALA interrupts the binding, adsorption, and entry stages of ZIKV replication cycle. The mechanism is probably that ALA disrupts membrane integrity of the virions to release ZIKV RNA, inhibiting viral infectivity. Further examination revealed that ALA inhibited DENV-2, HSV-1, influenza virus and SARS-CoV-2 infection dose-dependently. ALA is a promising broad-spectrum antiviral agent.
寨卡病毒（ZIKV）因其与包括小头畸形在内的严重先天性缺陷有关而引起全球关注。然而，目前还没有针对 ZIKV 感染的许可疫苗或药物。孕妇对治疗的需求最大，因此药物安全性至关重要。α-亚麻酸（ALA）是一种多不饱和ω-3 脂肪酸，由于其潜在的药用特性，已被用作保健品和膳食补充剂。在这里，我们证明 ALA 可在不降低细胞活力的情况下抑制细胞中的 ZIKV 感染。添加时间测定表明，ALA 中断了 ZIKV 复制周期的结合、吸附和进入阶段。其机制可能是 ALA 破坏了病毒粒子的膜完整性以释放 ZIKV RNA，从而抑制病毒的感染力。进一步的研究表明，ALA 能剂量依赖性地抑制 DENV-2、HSV-1、流感病毒和 SARS-CoV-2 的感染。ALA 是一种很有前途的广谱抗病毒药物。
