Brind J L, Alani E, Wheatley V R, Orentreich N
Comp Biochem Physiol B. 1986;84(3):403-7. doi: 10.1016/0305-0491(86)90098-2.
External ear of male and female Syrian hamsters (Mesocricetus auratus) were extracted with hexane and separated by class on thin-layer chromatography (TLC). Separated lipid classes were eluted and saponified, and non-saponifiable lipids further characterized by TLC, gas-liquid chromatography (GLC), UV and IR spectroscopy and functional group analyses. Many sex differences were observed, most notably the presence of sex-specific sterols of males and females. Mature animals were found to have greater quantities of ear sebum, but the characteristic qualitative lipid profiles of each sex were apparent in immature animals.
提取雄性和雌性叙利亚仓鼠(金仓鼠)的外耳并用己烷萃取,然后通过薄层色谱法(TLC)按类别分离。分离出的脂质类别被洗脱并皂化,不可皂化脂质通过TLC、气液色谱法(GLC)、紫外和红外光谱以及官能团分析进一步表征。观察到许多性别差异,最显著的是雄性和雌性存在性别特异性固醇。发现成熟动物的耳皮脂含量更高,但每种性别的特征性脂质定性图谱在未成熟动物中就已明显。