一种使用单克隆抗体定量检测血浆中人类C5a的灵敏酶联免疫吸附测定法。
A sensitive ELISA for the quantitation of human C5a in blood plasma using a monoclonal antibody.
作者信息
Schulze M, Götze O
出版信息
Complement. 1986;3(1):25-39. doi: 10.1159/000467875.
PMID:3743034
Abstract
An ELISA for the quantitation of the C5a anaphylatoxin of complement in human plasma is presented which is based on the use of both a monoclonal antibody and polyclonal antibodies to C5a. Its detection limit is 1 ng C5a/ml plasma. It detects no C5a in fresh EDTA-plasma. Complete removal of C5 by an optimized precipitation step prior to the assay procedure is an essential feature of the method.
摘要
本文介绍了一种用于定量检测人血浆中补体C5a过敏毒素的酶联免疫吸附测定法（ELISA），该方法基于使用针对C5a的单克隆抗体和多克隆抗体。其检测限为1纳克C5a/毫升血浆。在新鲜的乙二胺四乙酸（EDTA）血浆中未检测到C5a。在测定程序之前通过优化的沉淀步骤完全去除C5是该方法的一个基本特征。
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