Würzner R, Orren A, Potter P, Morgan B P, Ponard D, Späth P, Brai M, Schulze M, Happe L, Götze O
Department of Immunology, University of Göttingen, Germany.
Clin Exp Immunol. 1991 Mar;83(3):430-7. doi: 10.1111/j.1365-2249.1991.tb05656.x.
Two sensitive sandwich ELISAs based on monoclonal antibodies directed to native C6 and C7 allowed the detection and quantitation of these complement proteins in 20 out of 37 serum samples from individuals who had previously been classified as deficient in these proteins as assessed by immunochemical and/or functional assays. Furthermore, serum from four C6-deficient and one combined C6-/C7-deficient individual showed an increase in the terminal complement complex (TCC) and a decrease in native C6 and C7 after complement activation as assayed by specific ELISAs. Despite their (incomplete) deficiencies, these individuals therefore possess functionally active terminal complement proteins with respect to their ability to generate the TCC. As these individuals have no history of a susceptibility to neisserial infections, even low concentrations of functionally active C6 and C7 may provide sufficient protection against those micro-organisms whose destruction requires TCC formation.
两种基于针对天然C6和C7的单克隆抗体的灵敏夹心酶联免疫吸附测定（ELISA），能够对37份血清样本中的20份进行这些补体蛋白的检测和定量，这些血清样本来自之前经免疫化学和/或功能测定被归类为这些蛋白缺乏的个体。此外，通过特异性ELISA检测发现，来自4名C6缺乏个体和1名C6/C7联合缺乏个体的血清在补体激活后，终末补体复合物（TCC）增加，天然C6和C7减少。尽管这些个体存在（不完全）缺陷，但就其产生TCC的能力而言，他们拥有功能活性的终末补体蛋白。由于这些个体没有感染奈瑟菌的易感性病史，因此即使是低浓度的功能活性C6和C7也可能为抵御那些需要形成TCC才能被破坏的微生物提供足够的保护。
