花生四烯酸对牛胚前孵化和后孵化发育的影响。

Effect of arachidonic acid on pre- and post-hatching bovine embryo development.

机构信息

Animal Reproduction Department, INIA, CSIC, Madrid 28040, Spain.

Department of Animal Selection and Reproduction, SERIDA, Gijón 33394, Spain.

出版信息

Reprod Fertil Dev. 2023 Jul;35(12):614-621. doi: 10.1071/RD23053.

DOI:10.1071/RD23053

PMID:37430407

Abstract

CONTEXT

Arachidonic acid (AA) is the precursor of prostaglandins, which may play autocrine roles during early embryo development.

AIMS

To test the developmental effects of addition of AA to pre- and post-hatching culture media on in vitro -produced bovine embryos.

METHODS

Pre-hatching effects of AA were tested by culturing bovine zygotes in synthetic oviductal fluid (SOF) supplemented with 100 or 333μM AA. Post-hatching effects of AA were tested by culturing Day 7 blastocysts in N2B27 supplemented with 5, 10, 20 or 100μM AA up to Day 12.

KEY RESULTS

Pre-hatching development to blastocyst was completely abrogated at 333μM AA, whereas blastocyst rates and cell numbers were not altered at 100μM AA. Impaired post-hatching development was observed at 100μM AA, whereas no effect on survival rates was noted at 5, 10 and 20μM AA. However, a significant reduction in Day 12 embryo size was observed at 10 and 20μM AA. Hypoblast migration, epiblast survival and formation of embryonic-disc-like structures were unaffected at 5-10μM AA. AA exposure downregulated the genes PTGIS , PPARG , LDHA and SCD in Day 12 embryos.

CONCLUSIONS

Pre-hatching embryos are mostly irresponsive to AA, whereas AA was observed to have negative effects during early post-hatching development.

IMPLICATIONS

AA does not improve in vitro bovine embryo development and is not required up to early post-hatching stages.

摘要

背景

花生四烯酸（AA）是前列腺素的前体，在早期胚胎发育过程中可能发挥自分泌作用。

目的

检测在体外生产的牛胚胎的预孵化和孵化后培养基中添加 AA 的发育效果。

方法

通过在含有 100 或 333μM AA 的合成输卵管液（SOF）中培养牛受精卵来测试 AA 的预孵化作用。通过在 N2B27 中培养第 7 天的囊胚，并在第 12 天添加 5、10、20 或 100μM AA 来测试 AA 的孵化后作用。

结果

在 333μM AA 时，完全阻断了预孵化胚胎的囊胚发育，而在 100μM AA 时囊胚率和细胞数量没有改变。在 100μM AA 时观察到孵化后发育受损，而在 5、10 和 20μM AA 时未观察到对存活率的影响。然而，在 10 和 20μM AA 时，观察到 Day 12 胚胎大小显著减小。在 5-10μM AA 时，下胚层迁移、上胚层存活和胚胎盘样结构的形成不受影响。AA 暴露下调了 Day 12 胚胎中 PTGIS、PPARG、LDHA 和 SCD 的基因表达。