Temperate Tree Fruit and Vegetable Research Unit, USDA-ARS, Prosser, WA 99350.
Department of Entomology, Washington State University, Pullman, WA 99164.
Plant Dis. 2023 Dec;107(12):3958-3966. doi: 10.1094/PDIS-04-23-0769-RE. Epub 2023 Dec 12.
The beet leafhopper is an important pest of agricultural crops in the United States, where it transmits beet curly top virus, beet leafhopper-transmitted virescence agent phytoplasma, and to numerous crops, affecting yield and quality. Each of these pathogens have been linked to serious disease outbreaks within Washington State in the past century. To mitigate the risk of disease, growers target the beet leafhopper in their insect pest management programs. Knowledge of pathogen prevalence in beet leafhopper populations could help growers make better management decisions, but timely diagnostics is required. Four new assays were developed for the rapid detection of the beet leafhopper-associated pathogens. These include two assays that detect Beet leafhopper transmitted virescence agent (a PCR and a real-time PCR SYBR green assay), a duplex PCR assay that simultaneously detects beet curly top virus and , and a multiplex real-time PCR assay for the simultaneous detection of all three pathogens. The screening of dilution series generated from plant total nucleic acid extracts with these new assays typically led to detection at levels 10- to 100-fold more sensitive than the conventional PCR assays currently used. These new tools will allow the rapid detection of beet leafhopper-associated pathogens in both plant and insect specimens and will have the potential to be used in diagnostic laboratories seeking to disseminate fast and accurate results to growers for implementation in their insect pest monitoring programs.
标题：美国重要的农业害虫——斑须蝽
摘要：斑须蝽是美国农业作物的重要害虫，它传播甜菜曲顶病毒、甜菜叶蝉传播的黄化植原体以及，对众多作物造成影响，降低产量和品质。在过去的一个世纪里，这些病原体中的每一种都曾导致华盛顿州发生严重的疾病爆发。为了降低疾病风险，种植者在其虫害管理计划中针对斑须蝽。了解斑须蝽种群中病原体的流行情况可以帮助种植者做出更好的管理决策，但需要及时进行诊断。本研究开发了 4 种新的检测方法，用于快速检测与斑须蝽相关的病原体。其中包括 2 种检测 Beet leafhopper transmitted virescence agent 的方法（PCR 和实时荧光定量 PCR SYBR 绿法）、一种同时检测甜菜曲顶病毒和 的双重 PCR 方法，以及一种同时检测所有三种病原体的多重实时荧光定量 PCR 方法。使用这些新方法对植物总核酸提取物进行稀释系列的筛选，通常可以检测到比目前常用的常规 PCR 方法灵敏 10-100 倍的水平。这些新工具将能够快速检测植物和昆虫样本中的斑须蝽相关病原体，并有可能在诊断实验室中使用，以便向种植者快速准确地传播结果，从而实施在其虫害监测计划中。
