Crosson C E, Klyce S D, Bazan H E, Bazan N G
Curr Eye Res. 1986 Jul;5(7):535-41. doi: 10.3109/02713688608996377.
Tumor-promoting phorbol esters have been shown to modulate a number of physiological events in various cell types, and these events are associated with the activation of protein kinase C. The purpose of this study was to examine the effects of phorbol ester stimulation on protein kinase C activity and epithelial ion transport in the Cl- secreting rabbit corneal epithelium. We report here that nanomolar concentrations of tumor-promoting phorbol ester, 12-0-tetradecanoyl-phorbol-13 acetate (TPA), stimulate active ion transport via a putative receptor located in the corneal epithelium. This stimulation is a Cl- -dependent process, but is independent of beta-adrenergic receptor activation and prostaglandin formation. Biochemical data support the idea that TPA activates Ca++/phospholipid-dependent protein kinase C in the corneal epithelium. Therefore, we suggest that protein kinase C may have a role in the regulation of membrane Cl- transport in the mammalian corneal epithelium.
促肿瘤佛波酯已被证明可调节多种细胞类型中的一些生理事件，并且这些事件与蛋白激酶C的激活有关。本研究的目的是检测佛波酯刺激对分泌氯离子的兔角膜上皮中蛋白激酶C活性和上皮离子转运的影响。我们在此报告，纳摩尔浓度的促肿瘤佛波酯12-O-十四酰佛波醇-13-乙酸酯（TPA）通过位于角膜上皮中的假定受体刺激主动离子转运。这种刺激是一个依赖氯离子的过程，但不依赖于β-肾上腺素能受体激活和前列腺素形成。生化数据支持TPA激活角膜上皮中钙/磷脂依赖性蛋白激酶C的观点。因此，我们认为蛋白激酶C可能在哺乳动物角膜上皮膜氯离子转运的调节中起作用。
