Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, Oslo, Norway.
Department of Endodontics, Institute of Clinical Dentistry, University of Oslo, Oslo, Norway.
Clin Oral Investig. 2023 Sep;27(9):5203-5215. doi: 10.1007/s00784-023-05139-3. Epub 2023 Jul 11.
This study aimed to identify disease-related markers in persistent apical periodontitis (PAP) biopsies and examine whether these were associated with comorbidities like rheumatoid arthritis (RA) and cardiovascular diseases (CVD).
The levels of the cytokines/chemokines GM-CSF, IFN-γ, IL-2, IL-6, IL-9, IL-10, IL-13, IL-15, IL-17E/IL-25, IL-21, IL-23, IL-27, IL-28A/IFN -λ2, IL-33, MIP-3α/CCL20, and TNF-α were determined in lesions from patients with PAP (n = 20) and compared to healthy bone samples (n = 20).
We identified eleven cytokines to be differently expressed, and among them, IL-2, IL-6, IL-17E, IL-21, and IL-27 appeared to drive the discrepancy between the disease and healthy groups. The levels of T follicular helper (Tfh) cell promoting cytokines (IL-21, IL-6, IL-27) were enhanced while T helper (Th) 1 cell promoting cytokine (IL-2), Th2 cell promoting cytokine (IL-13), and Th17 cell promoting cytokine (IL-17E) were reduced in the PAP group. The data also indicate that Tfh cell differentiation (IL-21), along with Th1 (GM-CSF, IFNγ), Th2 (IL-13), and Th17 (GM-CSF) cell differentiation, might be increased in the subpopulation of patients suffering from RA, whereas no differences were found in patients with CVD.
Levels of cytokines/chemokines in PAP were identified, and cluster analyzes indicated that these markers may be associated with the differentiation of different T cell populations. Patients with PAP and RA comorbidities showed elevated levels of markers reinforcing this association.
Molecular analyses of PAP may result in identification of prognostic markers.
本研究旨在鉴定持续性根尖周炎（PAP）活检中的疾病相关标志物，并研究这些标志物是否与类风湿关节炎（RA）和心血管疾病（CVD）等合并症相关。
测定了患者（n=20）PAP 病变和健康骨样本（n=20）中细胞因子/趋化因子 GM-CSF、IFN-γ、IL-2、IL-6、IL-9、IL-10、IL-13、IL-15、IL-17E/IL-25、IL-21、IL-23、IL-27、IL-28A/IFN-λ2、IL-33、MIP-3α/CCL20 和 TNF-α 的水平，并将其与健康骨样本进行了比较。
我们鉴定出 11 种细胞因子表达不同，其中 IL-2、IL-6、IL-17E、IL-21 和 IL-27 似乎是导致疾病组与健康组差异的原因。滤泡辅助性 T（Tfh）细胞促进细胞因子（IL-21、IL-6、IL-27）水平升高，而辅助性 T（Th）1 细胞促进细胞因子（IL-2）、Th2 细胞促进细胞因子（IL-13）和 Th17 细胞促进细胞因子（IL-17E）水平降低。数据还表明，在患有 RA 的患者亚群中，Tfh 细胞分化（IL-21）以及 Th1（GM-CSF、IFNγ）、Th2（IL-13）和 Th17（GM-CSF）细胞分化可能增加，而在患有 CVD 的患者中则没有差异。
鉴定了 PAP 中的细胞因子/趋化因子水平，聚类分析表明这些标志物可能与不同 T 细胞群的分化有关。患有 PAP 和 RA 合并症的患者显示出升高的标志物水平，进一步证实了这种关联。
对 PAP 的分子分析可能会鉴定出预后标志物。
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