Lyles J M, Weill C L
Dev Neurosci. 1986;8(1):44-52. doi: 10.1159/000112240.
Glucose 6-phosphate dehydrogenase (G6PDH) activity was examined in the developing embryonic chick in brachial and lumbar spinal cord and pectoral and leg muscle. Enzyme activity was generally highest at the earliest stage examined, embryonic day 5. The developmental profiles for G6PDH activity in the two muscles were similar: a sharp initial decrease occurred between days 5 and 9, with relatively low levels present by day 18; peaks of G6PDH activity at days 12 and 16 were more prominent in leg muscle. Similar levels of G6PDH were also detected in spinal cord with the developmental profile in the brachial spinal cord resembling that seen in muscle. In lumbar spinal cord, initial G6PDH activity was lower than in brachial spinal cord; the developmental profile, however, resembled that seen in the brachial spinal cord, with an initial drop in enzyme activity seen between days 5 and 7. Neural regulation of G6PDH activity in mature muscle is believed to repress enzyme synthesis. The drop in G6PDH activity observed in embryonic spinal cord and muscle between days 5 and 9 coincides with the initiation of functional neuromuscular contacts. Hence, the normal regulation of G6PDH during embryonic development may involve the repression of G6PDH in spinal cord neurons and muscle, possibly effected by their interaction.
对发育中的胚胎小鸡的臂部和腰部脊髓以及胸肌和腿部肌肉中的葡萄糖-6-磷酸脱氢酶（G6PDH）活性进行了检测。在最早检测的阶段，即胚胎第5天，酶活性通常最高。两块肌肉中G6PDH活性的发育模式相似：在第5天到第9天之间出现急剧的初始下降，到第18天时水平相对较低；G6PDH活性在第12天和第16天的峰值在腿部肌肉中更为明显。在脊髓中也检测到了类似水平的G6PDH，臂部脊髓中的发育模式与肌肉中相似。在腰部脊髓中，初始G6PDH活性低于臂部脊髓；然而，发育模式与臂部脊髓相似，在第5天到第7天之间可见酶活性的初始下降。据信，成熟肌肉中G6PDH活性的神经调节会抑制酶的合成。在胚胎脊髓和肌肉中观察到的第5天到第9天之间G6PDH活性的下降与功能性神经肌肉接触的开始相吻合。因此，胚胎发育过程中G6PDH的正常调节可能涉及脊髓神经元和肌肉中G6PDH的抑制，可能是由它们之间的相互作用所导致的。
