Doherty Daniel Z, Ghith Amna, Ho Ava, De Voss James J, Bell Stephen G
Department of Chemistry, University of Adelaide, Adelaide, SA, 5005, Australia.
School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland, 4067, Australia.
Chem Commun (Camb). 2023 Jul 27;59(61):9392-9395. doi: 10.1039/d3cc02312e.
Cholesterol catabolism is an important survival mechanism for the pathogenic . Various other mycobacteria degrade not only cholesterol but plant sterols such as sitosterol and campesterol. In this work we demonstrate that the cytochrome P450 (CYP) CYP125 enzyme family is capable of sitosterol and campesterol side-chain oxidation and activation in these bacteria. We also show that the CYP142 and CYP124 cholesterol hydroxylating enzyme families are significantly less active for sitosterol hydroxylation compared to CYP125 enzymes.
胆固醇分解代谢是病原体的一种重要生存机制。其他各种分枝杆菌不仅能降解胆固醇，还能降解植物甾醇，如谷甾醇和菜油甾醇。在这项研究中，我们证明细胞色素P450（CYP）CYP125酶家族能够在这些细菌中对谷甾醇和菜油甾醇进行侧链氧化和激活。我们还表明，与CYP125酶相比，CYP142和CYP124胆固醇羟化酶家族对谷甾醇羟化的活性明显较低。
