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四膜虫的能量平衡以及进出腺苷酸库的物质通量。

The energy budget of Tetrahymena and the material fluxes into and out of the adenylate pool.

作者信息

Jauker F, Lades S, Nowack T

出版信息

Exp Cell Res. 1986 Sep;166(1):161-70. doi: 10.1016/0014-4827(86)90516-1.

DOI:10.1016/0014-4827(86)90516-1
PMID:3743653
Abstract

The material budget of the adenylate pool deals with all processes which physically establish and maintain this pool, while the energy budget is concerned with the intracompartmental ATP recycling. Both budgets were analysed in Tetrahymena thermophila exposed to various energy and material demands. Some of the general conclusions are: at a maximum growth rate the overall ATP consumption during one cell cycle is 10(-10) mol ATP; the contribution of osmoregulation and ciliary motion to the budget is about 1% each; at zero net growth, energy is consumed because of a continuous recycling of matter between the monomer and the polymer compartment. The rate of ATP production is about 1000-fold greater than the rate of adenylate monomer influx. The residence time of adenylate monomers within the pool is about 30 min, but for ATP molecules it is only 2 sec.

摘要

腺苷酸库的物质预算涉及所有实际建立和维持该库的过程,而能量预算则关注隔室内的ATP循环利用。对处于各种能量和物质需求下的嗜热四膜虫的这两种预算进行了分析。一些总体结论如下:在最大生长速率下,一个细胞周期内的总ATP消耗量为10^(-10) 摩尔ATP;渗透调节和纤毛运动对预算的贡献各约为1%;在净生长为零时,由于单体和聚合物隔室之间物质的持续循环利用,能量仍被消耗。ATP的产生速率比腺苷酸单体流入速率大约高1000倍。腺苷酸单体在库中的停留时间约为30分钟,但ATP分子的停留时间仅为2秒。

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