Yuen E, Brown R D, Kronenberg H, Rickard K A
Exp Hematol. 1986 Sep;14(8):771-5.
Agar cultures of human bone marrow cells stimulated by cord plasma or irradiated cord blood feeder layers demonstrated the presence of a multilineage hemopoietic growth factor in cord blood. When bone marrow cultures stimulated with giant cell tumor-conditioned medium (GCT-CM) were supplemented with this cord blood-derived growth factor, total colony numbers increased by more than 50% after day 23 and persisted in culture for approximately 40 days. Marrow cultures stimulated by the cord blood-derived growth factor formed colonies of neutrophils, monocyte-macrophages, eosinophils, mast cells, and a few colonies containing a mixed cell population. The results suggest that, while GCT-CM contains granulocyte-monocyte colony-stimulating factor (GM-CSF), cord blood contains a high concentration of a multilineage hemopoietic growth factor, which may be multi-CSF.
由脐血浆或经辐照的脐血饲养层刺激的人骨髓细胞琼脂培养物表明脐血中存在多谱系造血生长因子。当用巨细胞瘤条件培养基（GCT-CM）刺激的骨髓培养物补充这种源自脐血的生长因子时，在第23天后总集落数增加超过50%，并在培养中持续约40天。由源自脐血的生长因子刺激的骨髓培养物形成了中性粒细胞、单核细胞 - 巨噬细胞、嗜酸性粒细胞、肥大细胞的集落，以及一些含有混合细胞群体的集落。结果表明，虽然GCT-CM含有粒细胞 - 单核细胞集落刺激因子（GM-CSF），但脐血含有高浓度的多谱系造血生长因子，其可能是多集落刺激因子。
