Department of Pediatrics, Section of Developmental Biology, University of Colorado School of Medicine, Aurora, CO 80045, USA; Molecular and Cellular Life Sciences Program, University of Wyoming, Laramie, WY 82071, USA.
Department of Pediatrics, Section of Developmental Biology, University of Colorado School of Medicine, Aurora, CO 80045, USA.
STAR Protoc. 2023 Sep 15;4(3):102432. doi: 10.1016/j.xpro.2023.102432. Epub 2023 Jul 11.
Mammals typically have hundreds of distinct olfactory sensory neuron subtypes, each defined by expression of a specific odorant receptor gene, which undergo neurogenesis throughout life at rates that can depend on olfactory experience. Here, we present a protocol to quantify the birthrates of specific neuron subtypes via the simultaneous detection of corresponding receptor mRNAs and 5-ethynyl-2'-deoxyuridine. For preparation prior to beginning the protocol, we detail procedures for generating odorant receptor-specific riboprobes and experimental mouse olfactory epithelial tissue sections. For complete details on the use and execution of this protocol, please refer to van der Linden et al. (2020)..
哺乳动物通常具有数百种不同的嗅觉感觉神经元亚型，每种亚型都由特定的气味受体基因表达定义，这些基因在整个生命周期中都可以根据嗅觉经验进行神经发生。在这里，我们提出了一种通过同时检测相应的受体 mRNA 和 5-乙炔基-2'-脱氧尿苷来定量特定神经元亚型出生率的方案。在开始该方案之前的准备中，我们详细介绍了生成气味受体特异性核糖探针和实验小鼠嗅上皮组织切片的程序。有关该方案使用和执行的完整详细信息，请参阅 van der Linden 等人（2020 年）。。
