多克隆抗电鳗烟碱样受体抗血清与电鳐烟碱样受体的相互作用。

Interactions of polyclonal anti-Electrophorus nicotinic receptor antisera with Torpedo nicotinic receptor.

作者信息

Lukas R J

出版信息

Int J Biochem. 1986;18(7):609-15. doi: 10.1016/0020-711x(86)90290-9.

Abstract

Polyclonal antisera raised against solubilized and purified nicotinic acetylcholine receptor (nAcChoR) from Electrophorus electroplax and a polyclonal anti-alpha-bungarotoxin antiserum have been studied by the use of four different radioimmunoassay protocols. The results indicate unique sensitivities of different assay techniques in analysis of antibody-antigen interactions, and serve as a model for immunological study of other integral membrane proteins.

摘要

利用四种不同的放射免疫分析方法，对用从电鳗（Electrophorus electroplax）中溶解并纯化的烟碱型乙酰胆碱受体（nAcChoR）制备的多克隆抗血清以及一种抗α-银环蛇毒素多克隆抗血清进行了研究。结果表明不同的分析技术在分析抗体-抗原相互作用时具有独特的灵敏度，并且为其他整合膜蛋白的免疫学研究提供了一个模型。