在体条件下对小鼠视网膜中蛋白质稳定性的研究方法。

Methods for In Vivo Characterization of Proteostasis in the Mouse Retina.

机构信息

Department of Ophthalmology, University of Florida, Gainesville, FL, USA.

Department of Pharmacology and Therapeutics, University of Florida, Gainesville, FL, USA.

出版信息

Adv Exp Med Biol. 2023;1415:383-387. doi: 10.1007/978-3-031-27681-1_56.

DOI:10.1007/978-3-031-27681-1_56

PMID:37440061

Abstract

An increasing number of studies connect inherited and age-related retinal degenerations with changes in the regulation of proteostasis. Here, we describe technical aspects of existing assays allowing to assess the status of the ubiquitin-proteasome system (UPS), changes in autophagy, and protein translation in mouse retina in vivo. These methods are helpful for the development and testing approaches to modulate proteostasis and delay vision loss.

摘要

越来越多的研究将遗传性和年龄相关性视网膜变性与蛋白质稳态调节的变化联系起来。在这里，我们描述了现有的评估泛素-蛋白酶体系统 (UPS) 状态、自噬变化和体内小鼠视网膜蛋白质翻译的测定方法的技术方面。这些方法有助于开发和测试调节蛋白质稳态和延缓视力丧失的方法。