• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

人眼腺源性间充质干细胞的分离、增殖与鉴定。

Human Lacrimal Gland Derived Mesenchymal Stem Cells - Isolation, Propagation, and Characterization.

机构信息

Centre for Ocular Regeneration, Professor Brien Holden Eye Research Centre, Champalimaud Translational Centre for Eye Research, L. V. Prasad Eye Institute, Hyderabad, Telangana, India.

Manipal Academy of Higher Education (MAHE), Manipal, Karnataka, India.

出版信息

Invest Ophthalmol Vis Sci. 2023 Jul 3;64(10):12. doi: 10.1167/iovs.64.10.12.

DOI:10.1167/iovs.64.10.12
PMID:37440263
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10353750/
Abstract

PURPOSE

The existing treatment options for dry eye disease (DED) due to lacrimal gland (LG) dysfunction are mainly palliative. Mesenchymal stem cells (MSCs) based therapies and 3D-LG organoids have been explored as a curative option for LG regeneration in animal models. Human LG epithelial cultures are previously established and, here, we aim to isolate and characterize the spindle-shaped cells obtained from primary human LG cultures in order to unveil its MSC property.

METHODS

Normal human lacrimal glands were obtained from individuals undergoing LG debulking surgery. The conditions for human LG-MSC culture were standardized to obtain pure population of LG-MSCs at passage 3. Population doubling time (PDT), expression of phenotypic markers, tri-lineage differentiation, colony forming potential, and gene expression analysis were carried out to assess the phenotypic and genotypic characteristics compared to bone marrow-MSCs (BM-MSCs).

RESULTS

Our data show that these spindle-shaped cells exhibit similar phenotypic expression, colony-forming ability, and trilineage differentiation like BM-MSCs. Moreover, the gene expression also did not show any significant difference, except for increased IL1-β in LG-MSCs. The LG-MSCs do not express any lacrimal epithelial markers unlike LG tissue.

CONCLUSIONS

This study reveals the first-time evidence for the presence of MSC population within the human LGs, and these cells might play a role in maintaining healthy microenvironment within normal LG and repair in diseased LGs.

摘要

目的

由于泪腺（LG）功能障碍导致的干眼症（DED）的现有治疗选择主要是姑息性的。间充质干细胞（MSCs）为基础的治疗和 3D-LG 类器官已被探索作为动物模型中 LG 再生的一种治疗选择。人类 LG 上皮细胞培养以前已经建立，在这里，我们旨在分离和鉴定从原代人 LG 培养物中获得的纺锤形细胞，以揭示其 MSC 特性。

方法

从接受 LG 切除术的个体中获得正常的人泪腺。将人 LG-MSC 培养的条件标准化，以获得第 3 代时的纯 LG-MSC 群体。通过细胞倍增时间（PDT）、表型标志物表达、三系分化、集落形成潜力和基因表达分析，与骨髓间充质干细胞（BM-MSCs）进行比较，评估其表型和基因型特征。

结果

我们的数据表明，这些纺锤形细胞表现出与 BM-MSCs 相似的表型表达、集落形成能力和三系分化。此外，基因表达也没有显示出任何显著差异，除了 LG-MSCs 中 IL1-β 的增加。LG-MSCs 不表达任何泪腺上皮标志物，与 LG 组织不同。

结论

这项研究首次证明了 MSC 群体存在于人 LG 中，这些细胞可能在维持正常 LG 内的健康微环境和在患病 LG 中修复方面发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/10353750/7ef03b8fb199/iovs-64-10-12-f007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/10353750/3a4fe0b69501/iovs-64-10-12-f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/10353750/e1e8a79d9aed/iovs-64-10-12-f002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/10353750/2d3e7fc3f58b/iovs-64-10-12-f003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/10353750/eb13705cf4dc/iovs-64-10-12-f004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/10353750/8c3d7cd633f6/iovs-64-10-12-f005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/10353750/e7fa5d9f06fc/iovs-64-10-12-f006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/10353750/7ef03b8fb199/iovs-64-10-12-f007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/10353750/3a4fe0b69501/iovs-64-10-12-f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/10353750/e1e8a79d9aed/iovs-64-10-12-f002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/10353750/2d3e7fc3f58b/iovs-64-10-12-f003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/10353750/eb13705cf4dc/iovs-64-10-12-f004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/10353750/8c3d7cd633f6/iovs-64-10-12-f005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/10353750/e7fa5d9f06fc/iovs-64-10-12-f006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b35/10353750/7ef03b8fb199/iovs-64-10-12-f007.jpg

相似文献

1
Human Lacrimal Gland Derived Mesenchymal Stem Cells - Isolation, Propagation, and Characterization.人眼腺源性间充质干细胞的分离、增殖与鉴定。
Invest Ophthalmol Vis Sci. 2023 Jul 3;64(10):12. doi: 10.1167/iovs.64.10.12.
2
The Influence of Oxygen on the Proliferative Capacity and Differentiation Potential of Lacrimal Gland-Derived Mesenchymal Stem Cells.氧气对泪腺来源间充质干细胞增殖能力和分化潜能的影响
Invest Ophthalmol Vis Sci. 2015 Jul;56(8):4741-52. doi: 10.1167/iovs.14-15475.
3
Development of lacrimal gland spheroids for lacrimal gland tissue regeneration.泪腺球体的开发用于泪腺组织再生。
J Tissue Eng Regen Med. 2018 Apr;12(4):e2001-e2009. doi: 10.1002/term.2631. Epub 2018 Jan 29.
4
Establishment of functional epithelial organoids from human lacrimal glands.从人泪腺建立功能性上皮类器官。
Stem Cell Res Ther. 2021 Apr 21;12(1):247. doi: 10.1186/s13287-021-02133-y.
5
Analysis of lacrimal gland derived mesenchymal stem cell secretome and its impact on epithelial cell survival.泪腺来源间充质干细胞分泌组分析及其对上皮细胞存活的影响。
Stem Cell Res. 2019 Jul;38:101477. doi: 10.1016/j.scr.2019.101477. Epub 2019 Jun 4.
6
Establishing and characterizing lacrispheres from human lacrimal gland for potential clinical application.从人泪腺建立并表征泪球体以用于潜在的临床应用。
Graefes Arch Clin Exp Ophthalmol. 2018 Apr;256(4):717-727. doi: 10.1007/s00417-018-3926-8. Epub 2018 Feb 17.
7
Effect of Dry Eye Disease on the Kinetics of Lacrimal Gland Dendritic Cells as Visualized by Intravital Multi-Photon Microscopy.眼干燥症对活体多光子显微镜下观察到的泪腺树突状细胞动力学的影响。
Front Immunol. 2020 Aug 12;11:1713. doi: 10.3389/fimmu.2020.01713. eCollection 2020.
8
Lacrimal Gland Repair Using Progenitor Cells.使用祖细胞修复泪腺。
Stem Cells Transl Med. 2017 Jan;6(1):88-98. doi: 10.5966/sctm.2016-0191. Epub 2016 Aug 15.
9
Single-Nuclei Characterization of Lacrimal Gland in Scopolamine-Induced Dry Eye Disease.单细胞核分析在东莨菪碱诱导的干眼疾病中的泪腺作用。
Invest Ophthalmol Vis Sci. 2024 Apr 1;65(4):46. doi: 10.1167/iovs.65.4.46.
10
HIF1α-mediated TRAIL Expression Regulates Lacrimal Gland Inflammation in Dry Eye Disease.HIF1α 介导的 TRAIL 表达调控干燥性眼病中的泪腺炎症。
Invest Ophthalmol Vis Sci. 2020 Jan 23;61(1):3. doi: 10.1167/iovs.61.1.3.

引用本文的文献

1
Mesenchymal Stem Cell-Sourced Exosomes as Potentially Novel Remedies for Severe Dry Eye Disease.间充质干细胞来源的外泌体作为重度干眼病潜在的新型治疗方法
J Ophthalmol. 2025 Jan 10;2025:5552374. doi: 10.1155/joph/5552374. eCollection 2025.
2
Application of Single Cell Type-Derived Spheroids Generated by Using a Hanging Drop Culture Technique in Various Disease Models: A Narrow Review.利用悬滴培养技术生成的单细胞来源球体在各种疾病模型中的应用：一个简要综述。
Cells. 2024 Sep 14;13(18):1549. doi: 10.3390/cells13181549.
3
Impact of inflammasomes on the ocular surface.

本文引用的文献

1
Expansion and characterization of human limbus-derived stromal/mesenchymal stem cells in xeno-free medium for therapeutic applications.用于治疗应用的无动物源培养基中扩增和鉴定人角膜缘基质/间充质干细胞。
Stem Cell Res Ther. 2023 Apr 15;14(1):89. doi: 10.1186/s13287-023-03299-3.
2
IL-1b in the Secretomes of MSCs Seeded on Human Decellularized Allogeneic Bone Promotes Angiogenesis.人去细胞同种异体骨来源骨髓间充质干细胞的细胞外囊泡中的白细胞介素-1β促进血管生成。
Int J Mol Sci. 2022 Dec 4;23(23):15301. doi: 10.3390/ijms232315301.
3
Lacrimal gland regeneration: The unmet challenges and promise for dry eye therapy.
炎症小体对眼表的影响。
Curr Opin Allergy Clin Immunol. 2024 Oct 1;24(5):368-374. doi: 10.1097/ACI.0000000000001004. Epub 2024 Jun 20.
4
Application progress of single-cell sequencing technology in mesenchymal stem cells research.单细胞测序技术在间充质干细胞研究中的应用进展
Front Cell Dev Biol. 2024 Jan 9;11:1336482. doi: 10.3389/fcell.2023.1336482. eCollection 2023.
泪腺再生：干眼症治疗的未满足需求和前景。
Ocul Surf. 2022 Jul;25:129-141. doi: 10.1016/j.jtos.2022.06.005. Epub 2022 Jun 23.
4
Safety and feasibility of mesenchymal stem cell therapy in patients with aqueous deficient dry eye disease.间充质干细胞治疗水样液缺乏性干眼症患者的安全性和可行性。
Ocul Surf. 2021 Jan;19:43-52. doi: 10.1016/j.jtos.2020.11.013. Epub 2020 Nov 28.
5
The Human Lacrimal Gland: Historical Perspectives, Current Understanding, and Recent Advances.人类泪腺：历史视角、当前认识和最新进展。
Curr Eye Res. 2020 Oct;45(10):1188-1198. doi: 10.1080/02713683.2020.1774065. Epub 2020 Jun 15.
6
MSC Transplantation Improves Lacrimal Gland Regeneration after Surgically Induced Dry Eye Disease in Mice.MSC 移植可改善手术诱导的干燥眼病小鼠的泪腺再生。
Sci Rep. 2019 Dec 4;9(1):18299. doi: 10.1038/s41598-019-54840-5.
7
Encapsulation of human limbus-derived stromal/mesenchymal stem cells for biological preservation and transportation in extreme Indian conditions for clinical use.在极端印度条件下，为临床使用而对人眼角膜缘来源的基质/间充质干细胞进行生物保存和运输的封装。
Sci Rep. 2019 Nov 18;9(1):16950. doi: 10.1038/s41598-019-53315-x.
8
Three Dimensional Culture of Potential Epithelial Progenitor Cells in Human Lacrimal Gland.人泪腺中潜在上皮祖细胞的三维培养
Transl Vis Sci Technol. 2019 Aug 30;8(4):32. doi: 10.1167/tvst.8.4.32. eCollection 2019 Jul.
9
Analysis of lacrimal gland derived mesenchymal stem cell secretome and its impact on epithelial cell survival.泪腺来源间充质干细胞分泌组分析及其对上皮细胞存活的影响。
Stem Cell Res. 2019 Jul;38:101477. doi: 10.1016/j.scr.2019.101477. Epub 2019 Jun 4.
10
Inflammatory response of mesenchymal stromal cells after in vivo exposure with selected trauma-related factors and polytrauma serum.体内暴露于选定创伤相关因素和多发伤血清后间充质基质细胞的炎症反应。
PLoS One. 2019 May 14;14(5):e0216862. doi: 10.1371/journal.pone.0216862. eCollection 2019.