State Key Laboratory of Food Science and Resources, School of Food Science and Technology, International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, 214122, China.
Institute of Animal Husbandry and Veterinary Science, Xinjiang Academy of Agriculture and Reclamation Sciences, Shihezi, 83200, China.
Talanta. 2023 Dec 1;265:124891. doi: 10.1016/j.talanta.2023.124891. Epub 2023 Jun 30.
Herein, a SiO@Ag NPs core/shell nanoparticles were synthesized to fabricate a surface-enhanced Raman spectroscopy (SERS) sensor for the simultaneous determination of histamine (HIS) and tyramine (TYR) based on specific aptamer recognition and ratiometric strategy. SiO@Ag NPs with 4-thiosaminophenol (4-ATP) and Nile blue A (NBA) molecules were used as an internal standard (IS) and labeled with aptamers corresponding to HIS and TYR, respectively. Raman reporter molecules ROX and Cy5 labeled complementary DNA (cDNA) were then hybridized with aptamers to form rigid double-stranded DNA. After the HIS and TYR were captured by their aptamers, resulting in the dissociation of cDNA and separated from the SERS substrate. Therefore, the SERS signal intensity at 1503 cm of ROX and 1358 cm of Cy5 tagged on the terminal of cDNA decreased with the concentration of HIS and TYR increasing, while the SERS signal intensity at 1079 cm of 4-APT and 592 cm of NBA on the substrate remain stable. Thus, the concentrations of HIS and TYR can be determined by the I/I and I/I values, respectively. This sensing strategy achieves a lower detection limit of 0.2 ng/mL for HIS and 0.05 ng/mL for TYR, respectively, demonstrating promising applications in sensitive detection of BAs in animal-derived foodstuff.
在此，我们合成了 SiO@Ag NPs 核/壳纳米粒子，以基于特异性适体识别和比率策略，构建表面增强拉曼光谱（SERS）传感器，用于同时测定组胺（HIS）和酪胺（TYR）。SiO@Ag NPs 用 4-巯基苯并噻唑（4-ATP）和尼罗蓝 A（NBA）分子作为内标（IS），并分别标记与 HIS 和 TYR 相对应的适体。然后，将拉曼报告分子 ROX 和 Cy5 标记的互补 DNA（cDNA）与适体杂交，形成刚性双链 DNA。当 HIS 和 TYR 被其适体捕获时，cDNA 与 SERS 基底解离并分离。因此，随着 HIS 和 TYR 浓度的增加，标记在 cDNA 末端的 ROX 的 1503cm-1 和 Cy5 的 1358cm-1 的 SERS 信号强度降低，而基底上 4-ATP 的 1079cm-1 和 NBA 的 592cm-1 的 SERS 信号强度保持稳定。因此，可以通过 I/I 和 I/I 值分别确定 HIS 和 TYR 的浓度。该传感策略分别实现了对 HIS 和 TYR 的检测限为 0.2ng/mL 和 0.05ng/mL，在动物源性食品中 BAs 的灵敏检测方面具有广阔的应用前景。
