Department of Chemistry, Seoul National University, Seoul, 08826, Republic of Korea.
Program in Nano Science and Technology, Graduate School of Convergence Science and Technology, Seoul National University, Suwon-Si, Gyeonggi-do, 16229, Republic of Korea.
Biosens Bioelectron. 2023 Oct 1;237:115518. doi: 10.1016/j.bios.2023.115518. Epub 2023 Jul 5.
Viruses have unique coat proteins that are genetically modifiable. Their surface can serve as a nano-template on which electroactive molecules are immobilized. In this study, we report filamentous bacteriophage as a backbone to which redox mediators are covalently and densely tethered, constructing redox nanowire, i.e. an electron conducting biomaterial. The highly ordered coat proteins of a filamentous bacteriophage provide flexible and biocompatible platform to constitute a biohybrid redox nanowire. Incorporating bacteriophage and redox molecules form an entangled assembly of nanowires enabling facile electron transfer. Electron transfer among the molecular mediators in the entangled assembly originates apparent electron diffusion of which the electron transfer rate is comparable to that observed in conventional redox polymers. Programming peptide terminals suggests further enhancement in electron mediation by increasing redox species mobility. In addition, the redox nanowire film functions as a favorable matrix for enzyme encapsulation. The stability of the enzymes entrapped in this unique matrix is substantially improved.
病毒具有独特的外壳蛋白，这些蛋白具有遗传可修饰性。其表面可以作为纳米模板，在其上固定电化学生物分子。在这项研究中，我们报告了丝状噬菌体作为一种骨架，其上共价且密集地连接了氧化还原介体，构建了氧化还原纳米线，即电子传导生物材料。丝状噬菌体高度有序的外壳蛋白为构成生物杂化氧化还原纳米线提供了灵活且生物相容的平台。将噬菌体和氧化还原分子结合形成纳米线的缠结组装，实现了电子的快速转移。分子介体在缠结组装中的电子转移起源于明显的电子扩散，其电子转移速率可与传统氧化还原聚合物中的观察结果相媲美。通过增加氧化还原物质的迁移率，肽末端的编程可以进一步增强电子介导作用。此外，氧化还原纳米线薄膜可用作酶包封的理想基质。包封在这种独特基质中的酶的稳定性得到了显著提高。
