Clinical Research Department, London School of Hygiene & Tropical Medicine, London, UK.
MRC Unit The Gambia at London School of Hygiene & Tropical Medicine, London, UK.
BMC Infect Dis. 2023 Jul 13;23(1):471. doi: 10.1186/s12879-023-08399-2.
The prevalence of sexually transmitted infections (STIs) in sub-Saharan Africa is poorly described. We aimed to determine the prevalence of five treatable STIs (Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Mycoplasma genitalium, Treponema pallidum) in a sample of Gambian women from the general population.
Archived specimens from 420 women aged 15 - 69 years living in The Gambia enrolled in a clinical trial of human papilloma virus vaccine schedules were tested in this study. Urine samples were tested for C. trachomatis, N. gonorrhoeae, T. vaginalis and M. genitalium using a commercially available, open-platform multiplex PCR kit. A fragment of the ompA gene was amplified from C. trachomatis-positive samples and sequenced. Serum samples were tested for T. pallidum using the Chembio DPP Syphilis Screen and Confirm test.
Overall, 41/420 (9.8%) women tested positive for at least one STI. 32 (7.6%), 9 (2.1%), 1 (0.2%), 1 (0.2%) and 0 (0.0%) tested positive for T. vaginalis, C. trachomatis, N gonorrhoeae, M. genitalium and T. pallidum, respectively. ompA gene sequence was available from five C. trachomatis infections: four were genovar D,one was genovar G and one was genovar F.
STIs are endemic in The Gambia. Monitoring systems should be established.
撒哈拉以南非洲性传播感染（STI）的流行情况描述不足。我们旨在确定在冈比亚普通人群中的 420 名 15-69 岁女性样本中，五种可治疗的 STI（沙眼衣原体、淋病奈瑟菌、阴道毛滴虫、生殖支原体、梅毒螺旋体）的流行率。
本研究中使用了商业上可用的开放式多重 PCR 试剂盒，对来自参加人乳头瘤病毒疫苗方案临床试验的 420 名年龄在 15-69 岁的冈比亚女性的存档标本进行检测。尿液样本用于检测沙眼衣原体、淋病奈瑟菌、阴道毛滴虫和生殖支原体。从沙眼衣原体阳性样本中扩增 ompA 基因片段并进行测序。使用 Chembio DPP 梅毒筛查和确认试验检测血清样本中的梅毒螺旋体。
总体而言，41/420（9.8%）名女性至少有一种 STI 呈阳性。32（7.6%）、9（2.1%）、1（0.2%）、1（0.2%）和 0（0.0%）名女性分别检测出阴道毛滴虫、沙眼衣原体、淋病奈瑟菌、生殖支原体和梅毒螺旋体阳性。从五例沙眼衣原体感染中获得了 ompA 基因序列：四例为 D 型，一例为 G 型，一例为 F 型。
STI 在冈比亚流行。应建立监测系统。
