Institute of Physical Culture Sciences, University of Szczecin, 70-453 Szczecin, Poland.
Department of General Surgery and Transplantology, Pomeranian Medical University in Szczecin, 70-111 Szczecin, Poland.
Int J Mol Sci. 2023 Jun 25;24(13):10603. doi: 10.3390/ijms241310603.
The risk of losing a transplanted organ is high, and non-invasive markers to warn of this phenomenon are still being sought. We investigated the impact of post-transplant microchimerism on the function of the transplanted kidney. The study included 100 kidney transplant recipients, mostly women. All transplanted organs were from opposite-sex deceased donors. Microchimerism was assessed using multiplex PCR. Male DNA was detected in all urine samples from female recipients and in 13/56 blood samples from female kidney recipients. Female DNA was found in 31/44 urine samples from male recipients, but in none of the blood samples. Microchimerism in the urine of female recipients correlated positively with blood urea (Rs = 0.45; = 5.84 × 10) and K ions (Rs = 0.29; = 0.03), while microchimerism in the blood of female recipients also correlated positively with blood urea (Rs = 0. 28; = 0.04), cystatin C (Rs = 0.31; = 0.02) and the number of incompatible HLA alleles (Rs = 0.42; = 0.01). A history of DGF was associated with higher urinary donor DNA concentrations in female recipients.: Post-transplant microchimerism may serve as a potential marker of chronic kidney rejection.
移植器官丢失的风险很高，目前仍在寻找能够提示这种现象的非侵入性标志物。我们研究了移植后微嵌合体对移植肾功能的影响。该研究纳入了 100 例肾移植受者，大多数为女性。所有移植器官均来自异性已故供者。采用多重 PCR 评估微嵌合体。在所有女性受者的尿液样本和 56 例女性肾受者的 13/56 份血样中均检测到了男性 DNA。在 31/44 例男性受者的尿液样本中发现了女性 DNA，但在任何血样中均未发现。女性受者尿液中的微嵌合体与血尿素（Rs = 0.45； = 5.84 × 10）和 K 离子（Rs = 0.29； = 0.03）呈正相关，而女性受者血液中的微嵌合体与血尿素（Rs = 0.28； = 0.04）、胱抑素 C（Rs = 0.31； = 0.02）和不相容 HLA 等位基因数（Rs = 0.42； = 0.01）也呈正相关。DGF 病史与女性受者尿液中供者 DNA 浓度较高有关。：移植后微嵌合体可能是慢性肾排斥的潜在标志物。
