State Key Laboratory of Crop Biology, Key Laboratory of Crop Biology and Genetic Improvement of Horticultural Crops in Huanghuai Region, Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production in Shandong, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271018, China.
Int J Mol Sci. 2023 Jul 3;24(13):11042. doi: 10.3390/ijms241311042.
Chilling causes a significant decline in photosynthesis in tomato plants. Tomato tryptophan decarboxylase gene 1 () is the first rate-limiting gene for melatonin (MT) biosynthesis and is involved in the regulation of photosynthesis under various abiotic stresses. However, it is not clear whether participates in the photosynthesis of tomato under chilling stress. Here, we obtained overexpression transgenic tomato seedlings, which showed higher mRNA abundance and MT content compared with the wild type (WT). The results showed that the overexpression of obviously alleviated the chilling damage to seedlings in terms of the lower electrolyte leakage rate and hydrogen peroxide content, compared with the WT after 2 d of chilling stress. Moreover, the overexpression of notably increased photosynthesis under chilling stress, which was related to the higher chlorophyll content, normal chloroplast structure, and higher mRNA abundance and protein level of Rubisco and RCA, as well as the higher carbon metabolic capacity, compared to the WT. In addition, we found that -overexpressing seedlings showed higher W (damage degree of OEC on the PSII donor side), φ (quantum yield for electron transport in the PSII reaction center), and PI (photosynthetic performance index) than WT seedlings after low-temperature stress, implying that the overexpression of decreased the damage to the reaction center and donor-side and receptor-side electron transport of PSII and promoted PSI activity, as well as energy absorption and distribution, to relieve the photoinhibition induced by chilling stress. Our results support the notion that plays a vital role in the regulation of photosynthesis under chilling stress.
低温胁迫导致番茄光合作用显著下降。番茄色氨酸脱羧酶基因 1（）是褪黑素（MT）生物合成的第一个限速基因，参与各种非生物胁迫下光合作用的调节。然而，目前尚不清楚是否参与了番茄在低温胁迫下的光合作用。本研究获得了过表达 基因的番茄转基因幼苗，与野生型（WT）相比，其 mRNA 丰度和 MT 含量更高。结果表明，与 WT 相比，过表达 明显减轻了幼苗在低温胁迫 2 天后的胁迫损伤，表现为较低的电解质渗透率和过氧化氢含量。此外，过表达 明显提高了低温胁迫下的光合作用，这与更高的叶绿素含量、正常的叶绿体结构以及 Rubisco 和 RCA 的更高的 mRNA 丰度和蛋白水平有关，同时伴随着更高的碳代谢能力。此外，我们发现，与 WT 幼苗相比，过表达 幼苗在低温胁迫后具有更高的 W（PSII 供体侧 OEC 的损伤程度）、φ（PSII 反应中心电子传递的量子产率）和 PI（光合性能指数），这表明过表达 降低了 PSII 反应中心和供体侧及受体侧电子传递的损伤，促进了 PSI 活性以及能量的吸收和分配，从而缓解了低温胁迫引起的光抑制。我们的研究结果支持这样一种观点，即 在低温胁迫下的光合作用调节中起着至关重要的作用。
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