在进行高效液相色谱分析之前，采用凝胶渗透法在线去除血浆样品中的蛋白质。
Gel permeation for the on line removal of proteins from plasma samples prior to HPLC.
作者信息
Kraak J C, Kuiper K, Ruyter E
出版信息
Int J Environ Anal Chem. 1986;25(1-3):209-20. doi: 10.1080/03067318608077089.
PMID:3744656
Abstract
Gel permeation chromatography (GPC) has been investigated for the on line removal of proteins from plasma samples prior to their analysis by HPLC. The results show that GPC is a mild and effective way to remove proteins from plasma samples. It can very well be coupled on line to HPLC, providing the solutes are suitable for preconcentration on the analytical column itself or on a small pre-column, after the GPC. Under these conditions excellent reproducibility and accuracy can be obtained.
摘要
凝胶渗透色谱法（GPC）已被用于在血浆样品通过高效液相色谱（HPLC）分析之前在线去除蛋白质。结果表明，GPC是从血浆样品中去除蛋白质的一种温和且有效的方法。它可以很好地与HPLC在线联用，前提是溶质适合在GPC之后在分析柱本身或小型预柱上进行预浓缩。在这些条件下，可以获得出色的重现性和准确性。
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