Department of Food Analysis and Environmental Protection, Faculty of Chemical Technology and Engineering, Bydgoszcz University of Science and Technology, 85-326 Bydgoszcz, Poland.
Molecules. 2023 Jun 25;28(13):4981. doi: 10.3390/molecules28134981.
The fatty acid profile of cheese influences its sensory parameters, such as color, texture, or flavor. Examining the fatty acid profile also helps to assess the nutritional value of the cheese that is being tested. However, the determination of fatty acids in cheese samples is a multi-stage and time-consuming task. In addition, large amounts of toxic organic solvents are used to prepare samples for analysis purposes. This paper presents the results of a study to determine the fatty acid profile of yellow cheese samples. Six different methods of sample preparation were compared for analysis purposes. The profile of fatty acids was determined using gas chromatography with flame ionization detection (GC-FID). The study showed significant differences ( > 0.05) in the resulting fatty acid profile between the methods used. It was found that the most reliable fatty acid profile results were obtained using methods derived from the Folch method. In addition, tools such as the Analytical Eco-Scale tool and the Analytical Greenness Metric for Sample Preparation (AGREEprep) tool were used to assess the greenness of the methods used. In the case of the Analytical Eco-Scale tool, all six methods scored 'acceptable green analysis' with scores ranging from 61 to 73. However, an evaluation of methods using the AGREEprep metric showed that the results of the methods (0.13-0.27) did not show the "greenness" of the analytical methods.
奶酪的脂肪酸组成会影响其感官参数，如颜色、质地或风味。检查脂肪酸组成还有助于评估所测试奶酪的营养价值。然而，奶酪样品中脂肪酸的测定是一个多阶段且耗时的任务。此外，大量有毒的有机溶剂被用于为分析目的制备样品。本文介绍了一项测定黄奶酪样品脂肪酸组成的研究结果。为了分析目的，比较了六种不同的样品制备方法。使用带有火焰离子化检测的气相色谱法（GC-FID）来确定脂肪酸的图谱。研究表明，所使用的方法之间的脂肪酸图谱存在显著差异（>0.05）。结果表明，使用源自 Folch 方法的方法可以获得最可靠的脂肪酸图谱结果。此外，还使用了 Analytical Eco-Scale 工具和 Analytical Greenness Metric for Sample Preparation（AGREEprep）工具来评估所使用方法的绿色程度。在 Analytical Eco-Scale 工具的情况下，所有六种方法的得分为“可接受的绿色分析”，分值范围为 61 到 73。然而，使用 AGREEprep 度量标准对方法进行评估表明，方法的结果（0.13-0.27）并没有显示出分析方法的“绿色”程度。
