Institute of Experimental Pharmacology and Toxicology, Centre of Experimental Medicine SAS, 841 04 Bratislava, Slovakia.
Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Malá Hora 10701/4A, 036 01 Martin, Slovakia.
Molecules. 2023 Jun 28;28(13):5053. doi: 10.3390/molecules28135053.
extract (RSE) is mostly known for its adaptogen properties, but not for its antiarthritic activities, therefore monotherapy and combination with low-dose methotrexate (MTX) was studied. The collagen-induced arthritis (CIA) model was used to measure the functional score, and the change in hind paw volume (HPV). Both parameters had significant antiarthritic effects. Based on these preliminary results, an adjuvant arthritis (AA) model was further applied to assess another parameters. The experiment included these animal groups: healthy controls, untreated AA, AA administered with RSE (150 mg/kg b.w. daily, ), AA administered by MTX (0.3 mg/kg b.w. twice a week, ), and AA treated with the combination of RSE+MTX. The combination of RSE+MTX significantly reduced the HPV and increased the body weight. The combination significantly decreased HPV when compared to MTX monotherapy. The plasmatic levels of inflammatory markers (IL-6, IL-17A, MMP-9 and CRP) were significantly decreased by MTX+RSE treatment. The RSE monotherapy didn't influence any of the inflammatory parameters studied. In CIA, the RSE monotherapy significantly decreased the arthritic parameters studied. In summary, the combination of RSE and sub-therapeutic MTX was significantly effective in AA by improving inflammatory and arthritic parameters.
红景天提取物（RSE）主要以其适应原特性而闻名，但尚未发现其具有抗关节炎活性，因此研究了其单药治疗和与低剂量甲氨蝶呤（MTX）联合治疗的效果。本研究采用胶原诱导关节炎（CIA）模型来评估功能评分和后爪容积（HPV）的变化，这两个参数都具有显著的抗关节炎作用。基于这些初步结果，进一步应用佐剂关节炎（AA）模型来评估其他参数。实验包括以下动物组：健康对照组、未治疗的 AA 组、给予 RSE（150mg/kg bw 每日）的 AA 组、给予 MTX（0.3mg/kg bw 每周两次）的 AA 组以及给予 RSE+MTX 联合治疗的 AA 组。与 MTX 单药治疗相比，RSE+MTX 联合治疗显著降低了 HPV 和增加了体重。与 MTX 单药治疗相比，RSE+MTX 联合治疗显著降低了 HPV。RSE 单药治疗对研究的任何炎症参数均无影响。在 CIA 中，RSE 单药治疗显著降低了所研究的关节炎参数。总之，RSE 和亚治疗剂量 MTX 的联合治疗通过改善炎症和关节炎参数，对 AA 具有显著的治疗效果。
