Zheng Xiang, Zhang Bei, Pan Ni, Cheng Xue, Lu Wei
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
College of Life Sciences, Westlake University, Hangzhou 310000, China.
Plants (Basel). 2023 Jun 22;12(13):2413. doi: 10.3390/plants12132413.
Hydrogen sulfide (HS) plays prominent multifunctional roles in the mediation of various physiological processes and stress responses to plants. In this study, hydroponic experiments were carried out to explore the effects of NaHS pretreatment on the growth of wheat ( L.) under 50 μM cadmium (Cd). Compared with Cd treatment alone, 50 μM NaHS pretreatment increased the plant height, soluble sugar content of shoots and roots, and dry weight of shoots and roots under Cd stress, while the Cd concentration of shoots and roots was significantly reduced by 18.1% and 25.9%, respectively. Meanwhile, NaHS pretreatment protected the photosynthetic apparatus by increasing the net photosynthetic rate and PSII electron transportation rate of wheat leaves under Cd stress. NaHS pretreatment significantly increased the soluble sugar content to maintain the osmotic pressure balance of the leaf cells. The gene expression results associated with photosynthetic carbon assimilation and sucrose synthesis in wheat leaves suggested that the NaHS pretreatment significantly up-regulated the expression of , , and , while it down-regulated the expression of , , , and . In summary, NaHS pretreatment improved the resistance of wheat seedlings under Cd stress by increasing the rate of photosynthesis and regulating the expression of genes related to sugar metabolism.
硫化氢（HS）在介导植物的各种生理过程和应激反应中发挥着重要的多功能作用。在本研究中，进行了水培实验，以探究50 μM镉（Cd）胁迫下NaHS预处理对小麦（L.）生长的影响。与单独的Cd处理相比，50 μM NaHS预处理在Cd胁迫下增加了植株高度、地上部和根部的可溶性糖含量以及地上部和根部的干重，而地上部和根部的Cd浓度分别显著降低了18.1%和25.9%。同时，NaHS预处理通过提高Cd胁迫下小麦叶片的净光合速率和PSII电子传递速率来保护光合机构。NaHS预处理显著增加了可溶性糖含量，以维持叶细胞的渗透压平衡。与小麦叶片光合碳同化和蔗糖合成相关的基因表达结果表明，NaHS预处理显著上调了、和的表达，而下调了、、和的表达。综上所述，NaHS预处理通过提高光合速率和调节糖代谢相关基因的表达，提高了Cd胁迫下小麦幼苗的抗性。
