Wang Shijia, Li Ruimei, Zhou Yangjiao, Fernie Alisdair R, Ding Zhongping, Zhou Qin, Che Yannian, Yao Yuan, Liu Jiao, Wang Yajie, Hu Xinwen, Guo Jianchun
College of Life Sciences, Hainan University, Haikou 570228, China.
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
Plants (Basel). 2023 Jul 3;12(13):2529. doi: 10.3390/plants12132529.
Plant pectin methylesterases (PMEs) play crucial roles in regulating cell wall modification and response to various stresses. Members of the PME family have been found in several crops, but there is a lack of research into their presence in cassava (), which is an important crop for world food security. In this research, 89 genes were identified in cassava that were separated into two types (type-Ⅰ and type-Ⅱ) according to the existence or absence of a pro-region (PMEI domain). The gene members were unevenly located on 17 chromosomes, with 19 gene pairs being identified that most likely arose via duplication events. The could be divided into ten sub-groups in type-Ⅰ and five sub-groups in type-Ⅱ. The motif analysis revealed 11 conserved motifs in type-Ⅰ and 8 in type-Ⅱ . The number of introns in the CDS region of type-Ⅰ ranged between one and two, and the number of introns in type-Ⅱ ranged between one and nine. There were 21 type-Ⅰ and 31 type-Ⅱ that contained signal peptides. Most of the type-Ⅰ had two conserved "RK/RLL" and one "FPSWVS" domain between the pro-region and the PME domain. Multiple stress-, hormone- and tissue-specific-related -acting regulatory elements were identified in the promoter regions of genes. A total of five co-expressed genes (, , , and ) were filtered from different abiotic stresses via the use of UpSet Venn diagrams. The gene expression pattern analysis revealed that the expression of was positively correlated with the degree of cassava postharvest physiological deterioration (PPD). The expression of this gene was also significantly upregulated by 7% PEG and 14 °C low-temperature stress, but slightly downregulated by ABA treatment. The tissue-specific expression analysis revealed that and generally displayed higher expression levels in most tissues than the other co-expressed genes. In this study, we obtain an in-depth understanding of the cassava gene family, suggesting that could be a candidate gene associated with multiple abiotic tolerance.
植物果胶甲基酯酶（PMEs）在调节细胞壁修饰和应对各种胁迫方面发挥着关键作用。在几种作物中已发现PME家族成员，但对于它们在木薯（一种对世界粮食安全至关重要的作物）中的存在情况缺乏研究。在本研究中，在木薯中鉴定出89个基因，根据前体区域（PMEI结构域）的有无将其分为两种类型（Ⅰ型和Ⅱ型）。这些基因成员不均匀地分布在17条染色体上，鉴定出19对基因，它们很可能是通过复制事件产生的。Ⅰ型可分为十个亚组，Ⅱ型可分为五个亚组。基序分析揭示Ⅰ型中有11个保守基序，Ⅱ型中有8个。Ⅰ型CDS区域的内含子数量在1到2个之间，Ⅱ型的内含子数量在1到9个之间。有21个Ⅰ型和31个Ⅱ型含有信号肽。大多数Ⅰ型在其前体区域和PME结构域之间有两个保守的“RK/RLL”和一个“FPSWVS”结构域。在这些基因的启动子区域鉴定出多个与多种胁迫、激素和组织特异性相关的顺式作用调控元件。通过使用UpSet维恩图从不同非生物胁迫中筛选出总共5个共表达基因（、、、和）。基因表达模式分析表明，的表达与木薯采后生理劣变（PPD）程度呈正相关。该基因的表达也在7% PEG和14℃低温胁迫下显著上调7%，但在ABA处理下略有下调。组织特异性表达分析表明，和在大多数组织中通常比其他共表达基因显示出更高的表达水平。在本研究中，我们对木薯基因家族有了深入了解，表明可能是一个与多种非生物耐受性相关的候选基因。
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