Muñoz Valeria, Curillo Diana, Gómez Sebastián, Moreno-Peña Lissette, Peña Denisse, Chica Eduardo, Yánez Viviana, Sánchez-Timm Eduardo, Quito-Avila Diego F
Centro de Investigaciones Biotecnológicas del Ecuador (CIBE), Escuela Superior Politécnica del Litoral, ESPOL, Guayaquil 090101, Ecuador.
Facultad de Ciencias Agrícolas, Universidad de Cuenca, Campus Yanuncay, Av. October 12 and Diego de Tapia, Cuenca 010107, Ecuador.
Plants (Basel). 2023 Jul 6;12(13):2560. doi: 10.3390/plants12132560.
Babaco (), a fruit-bearing vegetatively propagated crop native to Ecuador, is appreciated for its distinctive flavor and nutritional properties. The aim of this research was to determine a functional protocol for tissue culture propagation of virus-free babaco plants including in vitro establishment, multiplication, rooting, and acclimation. First, symptomless babaco plants from a single commercial nursery were analyzed for virus detection and cared for using different disinfection treatments in the greenhouse to reduce contamination during the in vitro establishing step, and three cytokinins, 6-(γ,γ-Dimethylallylamino) purine (2IP), 6-Benzylaminopurine (BAP), and Thidiazuron (TDZ), were used to determine the best hormone for multiplication. The best treatment for plant disinfection was the weekly application of copper sulfate at the greenhouse and a laboratory disinfection using ethanol (EtOH) (70%), Clorox (2%), and a solution of povidone iodine (2.5%), with an 80% survival during in vitro plant establishment. TDZ showed a better multiplication rate when compared with other hormones, and 70% of the rooted plants were successfully acclimated at the greenhouse. Generated plants were virus-free when tested against babaco mosaic virus (BabMV) and papaya ringspot virus (PRSV), two of the most important viruses that can affect babaco. An efficient protocol to produce virus-free babaco plants was elaborated with an integrated use of viral diagnostic tools to ensure the production of healthy start material to farmers.
巴巴科（）是一种原产于厄瓜多尔的通过营养繁殖的结果作物，因其独特的风味和营养特性而受到青睐。本研究的目的是确定一种用于无病毒巴巴科植物组织培养繁殖的实用方案，包括离体建立、增殖、生根和驯化。首先，对来自单一商业苗圃的无症状巴巴科植物进行病毒检测分析，并在温室中采用不同的消毒处理进行养护，以减少离体建立阶段的污染。使用三种细胞分裂素，即6-(γ,γ-二甲基烯丙基氨基)嘌呤（2IP）、6-苄基氨基嘌呤（BAP）和噻苯隆（TDZ）来确定最佳增殖激素。植物消毒的最佳处理方法是在温室中每周施用硫酸铜，并在实验室中使用乙醇（EtOH）（70%）、高乐氏（2%）和聚维酮碘溶液（2.5%）进行消毒，离体植物建立期间的存活率为80%。与其他激素相比，TDZ显示出更好的增殖率，70%的生根植物在温室中成功驯化。当针对巴巴科花叶病毒（BabMV）和番木瓜环斑病毒（PRSV）这两种可影响巴巴科的最重要病毒进行检测时，所产生的植物无病毒。通过综合使用病毒诊断工具，精心制定了一种生产无病毒巴巴科植物的有效方案，以确保为农民提供健康的起始材料。
