Mosquera-Yuqui Francisco, Flores Francisco J, Moncayo Eduardo A, Garzón-Proaño Brighitte A, Méndez Miguel A, Guevara Fiama E, Quito-Avila Diego F, Viera William, Cornejo-Franco Juan F, Izquierdo Andrés R, Noceda Carlos
Grupo de Investigación y Desarrollo de la Biotecnología BioSin-Biociencias, Quito 170801, Ecuador.
Unidad de Citometría de Flujo y Biología Molecular, Departamento de Apoyo Diagnóstico, Hospital Oncológico Solón Espinosa Ayala, SOLCA Núcleo de Quito, Quito 170138, Ecuador.
Plants (Basel). 2022 Jun 22;11(13):1646. doi: 10.3390/plants11131646.
Babaco is a fast-growing herbaceous shrub with great commercial potential because of the organoleptic properties of its fruit. Babaco mosaic virus (BabMV) is a potexvirus in the family affecting babaco in all the provinces that produce this crop in Ecuador. BabMV was recently described but it has been affecting babaco for decades and, since many potexviruses are serologically indistinguishable, it may have been previously misidentified as papaya mosaic virus. Based on the coat protein (CP) gene, we aimed to study the distribution and epidemiological patterns of BabMV in babaco and chamburo over the years and to model its three-dimensional structure. Sequences of the CP were obtained from thirty-six isolates from plants collected in the main babaco-producing provinces of Ecuador between 2016 and 2021. The evolution rate of BabMV was estimated at 1.21 × 10 nucleotide substitutions site year and a time of origin of the most recent common ancestor around 1958.80. From molecular dynamics simulations, compared to other proteins of BabMV-RDRP, TGB1, and Alkb domain-the CP exhibited a higher flexibility with the C and N terminals as the most flexible regions. The reconstructed viral distribution provides dispersion patterns which have implications for control approaches of BabMV.
巴巴科是一种生长迅速的草本灌木，由于其果实的感官特性，具有巨大的商业潜力。巴巴科花叶病毒（BabMV）是一种隶属于该科的马铃薯X病毒属病毒，在厄瓜多尔所有种植这种作物的省份都对巴巴科造成影响。BabMV最近才被描述，但它已经影响巴巴科数十年了，而且由于许多马铃薯X病毒属病毒在血清学上难以区分，它之前可能被误鉴定为木瓜花叶病毒。基于外壳蛋白（CP）基因，我们旨在研究多年来BabMV在巴巴科和钱布罗中的分布及流行病学模式，并对其三维结构进行建模。CP序列是从2016年至2021年间在厄瓜多尔主要的巴巴科种植省份采集的植物中获得的36个分离株中获取的。BabMV的进化速率估计为1.21×10核苷酸替换位点/年，最近共同祖先的起源时间约为1958.80年。从分子动力学模拟来看，与BabMV的其他蛋白质（RNA依赖的RNA聚合酶、运动蛋白1和Alkb结构域）相比，CP表现出更高的灵活性，其C端和N端是最灵活的区域。重建的病毒分布提供了扩散模式，这对BabMV的控制方法具有启示意义。
