Davis W L, Nelson J A, Barry W H
Invest Radiol. 1986 Aug;21(8):626-30. doi: 10.1097/00004424-198608000-00005.
To quantify and characterize mechanisms of negative inotropic effects of ionic and nonionic angiographic contrast media, spontaneously contracting monolayers of cultured chick ventricular cells were studied using the Renografin-76 and iohexol as characteristic ionic and nonionic contrast agents. Utilizing microscopic video-motion detector techniques the effect of each contrast agent on contraction amplitude was measured. Also, the effect of changes in the ionized calcium concentration and osmolality induced by the contrast agents was evaluated. The study demonstrated a linear depression of contractility by increasing contrast concentrations. The negative inotropic effect of Renografin-76 was significantly greater than that of iohexol. Renografin-76 demonstrated significant calcium binding, whereas iohexol had none. Correction for calcium binding by Renografin-76 partially reversed the negative inotropic effect of this agent. Hypertonic sugar solutions of equivalent osmolality to the contrast agents demonstrated dose-related negative inotropic effects such that, in this preparation, calcium binding by Renografin-76 and its high osmolality accounted for all its negative inotropic effect. In contrast, iohexol demonstrated an intrinsic negative inotropic effect not completely explained by its osmolality.
为了量化和描述离子型和非离子型血管造影剂负性肌力作用的机制，我们使用泛影葡胺-76和碘海醇作为典型的离子型和非离子型造影剂，对培养的鸡心室细胞自发收缩单层进行了研究。利用显微视频运动检测技术，测量了每种造影剂对收缩幅度的影响。此外，还评估了造影剂引起的离子钙浓度和渗透压变化的影响。研究表明，随着造影剂浓度的增加，收缩力呈线性下降。泛影葡胺-76的负性肌力作用明显大于碘海醇。泛影葡胺-76显示出显著的钙结合，而碘海醇则没有。校正泛影葡胺-76的钙结合作用可部分逆转该药物的负性肌力作用。与造影剂等渗的高渗糖溶液显示出剂量相关的负性肌力作用，因此，在本实验制剂中，泛影葡胺-76的钙结合及其高渗性解释了其所有的负性肌力作用。相比之下，碘海醇显示出一种内在的负性肌力作用，其渗透压并不能完全解释这种作用。
