Department of Orthodontics, Beijing Stomatological Hospital, School of Stomatology, Capital Medical University, Beijing, China.
Oral Dis. 2024 May;30(4):2511-2522. doi: 10.1111/odi.14671. Epub 2023 Jul 13.
Exosomes derived from stem cells are a potential cell-free tool for tissue regeneration with therapeutic potential. However, its application in cementum repair is unclear. This study aimed to investigate the effect of human periodontal ligament stem cell-derived exosomes on the biological activity of cementoblasts, the main effector cells in cementum synthesis.
OCCM-30 cementoblasts were cultured with various human periodontal ligament stem cell-derived exosome concentrations. OCCM-30 cells proliferation, migration, and cementogenic mineralization were examined, along with the gene and protein expression of factors associated with cementoblastic mineralization.
Exosomal promoted the migration, proliferation, and mineralization of OCCM-30 cells. The exosome-treated group significantly increased the expression of cementogenic-related genes and proteins. Furthermore, the expression of p-PI3K and p-AKT was enhanced by exosome administration. Treatment with a PI3K/AKT inhibitor markedly attenuated the gene and protein expression of cementoblastic factors, and this effect was partially reversed by exosome administration.
Human periodontal ligament stem cell-derived exosomes can promote the activity of cementoblasts via the PI3K/AKT signaling pathway, providing a scientific basis for promoting the repair process in orthodontically induced inflammatory root resorption.
干细胞衍生的外泌体是一种具有治疗潜力的组织再生的潜在无细胞工具。然而，其在牙骨质修复中的应用尚不清楚。本研究旨在探讨人牙周膜干细胞衍生的外泌体对成牙骨质细胞生物学活性的影响，成牙骨质细胞是牙骨质合成的主要效应细胞。
用不同浓度的人牙周膜干细胞衍生的外泌体培养 OCCM-30 成牙骨质细胞。检测 OCCM-30 细胞的增殖、迁移和矿化特性，以及与成牙骨质矿化相关的因子的基因和蛋白表达。
外泌体促进了 OCCM-30 细胞的迁移、增殖和矿化。外泌体处理组显著增加了成牙骨质相关基因和蛋白的表达。此外，外泌体处理后 p-PI3K 和 p-AKT 的表达增强。用 PI3K/AKT 抑制剂处理显著减弱了成牙骨质因子的基因和蛋白表达，而外泌体处理部分逆转了这种作用。
人牙周膜干细胞衍生的外泌体可以通过 PI3K/AKT 信号通路促进成牙骨质细胞的活性，为促进正畸诱导的炎症性牙根吸收的修复过程提供了科学依据。
