Mikheev R K, Andreeva E N, Grigoryan O R, Sheremetyeva E V, Absatarova Yu S, Loginova E V
Endocrinology Research Centre.
Endocrinology Research Centre; Moscow State University of Medicine and Dentistry of A.I. Evdokimov.
Probl Endokrinol (Mosk). 2023 May 12;69(2):92-98. doi: 10.14341/probl13253.
One of the most dangerous reproductive pathologies is primary ovarian insufficiency (POI). Except manifestation in the age <40 years old it leads to demographical losses, decrease of chances for healthy aging. POI can be characterized as summary of secondary amenorrhea, total estrogenic deficiency and hypergonadotropic hypogonadism. Hence, POI has probably harmful effect on telomere length. Telomere length determining and sex steroid replacement therapy may be promising and effective to prevent decrease of life quality/ longevity among females with POI.
To evaluate features of replicative (telomere length) and biochemical (metabolic syndrome) markers among females with primary ovarian insufficiency.
Research has been provided in collaboration between Endocrinology Research Centre of the Russian Ministry of Health and Lomonosov Moscow State University Medical Research and Educational Centre in the period since 10.01.2021 until 01.08.2022. Females with non-iatrogenic hypergonadotropic hypogonadism caused by primary ovarian insufficiency (n=33); healthy females of reproductive age (18-49 y.o.; n=24). Patients have undergone laboratory genetic (leucocyte telomere length), biochemical analyses. DNA extraction - with Qiagen DNA blood mini kit (Germany).Leukocyte telomere length - with real-time polymerase chain reaction PCR (Flow-fish). Soft program IBM SPSS Statistics (version 26,0 for Windows).
Females with POI due to estrogenic deficiency have slightly shorter mean telomere length (10,0 [7,9-10,7] kB, than healthy females of reproductive age (10,8 [10,0-13,1] кБ, р<0,001). Females with POI due to estrogenic deficiency have higher chances for development of carbohydrate metabolism disturbances (prediabetes) (р<0,043), increasement of FSH level (р<0,001). FSH level correlates moderately and negatively (ρ=0,434) with leukocyte telomere length (р<0,001).
Female with POI and receiving sex steroid replacement therapy have decrease of telomere length and increase of chances for carbohydrate metabolism disturbances in opposite to healthy reproductive females.
最危险的生殖系统疾病之一是原发性卵巢功能不全（POI）。除了在40岁之前出现症状外，它还会导致人口统计学上的损失，降低健康衰老的几率。POI的特征可能是继发性闭经、完全雌激素缺乏和高促性腺激素性性腺功能减退。因此，POI可能对端粒长度有有害影响。确定端粒长度和进行性类固醇替代疗法可能是预防POI女性生活质量/寿命下降的有前景且有效的方法。
评估原发性卵巢功能不全女性的复制性（端粒长度）和生化（代谢综合征）标志物特征。
研究由俄罗斯卫生部内分泌研究中心和莫斯科国立罗蒙诺索夫大学医学研究与教育中心于2021年1月10日至2022年8月1日合作开展。患有原发性卵巢功能不全导致的非医源性高促性腺激素性性腺功能减退的女性（n = 33）；健康育龄女性（18 - 49岁；n = 24）。患者接受了实验室基因（白细胞端粒长度）、生化分析。DNA提取 - 使用Qiagen DNA血液微量试剂盒（德国）。白细胞端粒长度 - 使用实时聚合酶链反应PCR（Flow - fish）。软件程序为IBM SPSS Statistics（Windows版26.0）。
由于雌激素缺乏导致POI的女性平均端粒长度略短（10.0 [7.9 - 10.7] kB），低于健康育龄女性（10.8 [10.0 - 13.1] кБ，p < 0.001）。由于雌激素缺乏导致POI的女性发生碳水化合物代谢紊乱（糖尿病前期）的几率更高（p < 0.043），促卵泡生成素（FSH）水平升高（p < 0.001）。FSH水平与白细胞端粒长度呈中度负相关（ρ = 0.434）（p < 0.001）。
与健康育龄女性相反，接受性类固醇替代疗法的POI女性端粒长度缩短，发生碳水化合物代谢紊乱的几率增加。
