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INTRODUCTION

The number of neonatal cerebrospinal fluid (CSF) samples sent from the neonatal intensive care unit (NICU) for cytologic examination is rising, warranting accurate analysis and interpretation of the same. This study was taken up to assess the usefulness of CSF cell count and cytology in NICU settings, as it can be used even in a resource-limited setting.

AIM AND OBJECTIVE

To study the prevalence of cell count and cytologic changes in CSF from NICU and assess their usefulness in correlation to C-reactive protein, CSF neutrophil percentage, blood, CSF culture, and other biochemical parameters. 2) To correlate cell counts and cytology with age, period of gestation, presence, and absence of sepsis, seizures, intracranial hemorrhage, and their clinical follow-up.

MATERIALS AND METHODS

A retrospective study was done on neonatal CSF samples submitted for cytology over one year (January-December 2016) in the Department of Pathology. CSF cell counts were retrieved, and cytosmears were reviewed for cellularity, cell type, proportion, and background and correlated with the biochemical, microbiological, and clinicoradiological findings.

RESULTS

A total of 213 samples were included with 140 males and 73 females with an age range of 0-28 (mean: 7.3) days. The mean CSF cell count was 5.48/cu.mm (0-90 cells/cu.mm). The most frequent cytologic finding was occasional lymphocytes or acellular CSF (63.9%). The CSF leucocyte count and protein levels showed a significant correlation with s C-reactive protein. The CSF cytology showed a significant correlation between the age of the neonate and blood neutrophil percentage ( = 0.0158). History of intracranial hemorrhage showed a significantly higher frequency of the presence of red blood cells ( = 0.0147).

CONCLUSION

Accurate cell counts, cytology of neonatal CSF, and biochemical and microbiological workup can help diagnose and manage neonates in intensive care.

INTRODUCTION

AIM AND OBJECTIVE

To study the prevalence of cell count and cytologic changes in CSF from NICU and assess their usefulness in correlation to C-reactive protein, CSF neutrophil percentage, blood, CSF culture, and other biochemical parameters. 2) To correlate cell counts and cytology with age, period of gestation, presence, and absence of sepsis, seizures, intracranial hemorrhage, and their clinical follow-up.

MATERIALS AND METHODS

RESULTS

CONCLUSION

Accurate cell counts, cytology of neonatal CSF, and biochemical and microbiological workup can help diagnose and manage neonates in intensive care.

引言

新生儿重症监护病房（NICU）送检进行细胞学检查的新生儿脑脊液（CSF）样本数量不断增加，因此需要对其进行准确的分析和解读。本研究旨在评估CSF细胞计数和细胞学检查在NICU环境中的实用性，因为即使在资源有限的情况下也可使用。

目的

1）研究NICU中CSF细胞计数和细胞学变化的发生率，并评估它们与C反应蛋白、CSF中性粒细胞百分比、血液、CSF培养及其他生化参数相关的实用性。2）将细胞计数和细胞学与年龄、孕周、败血症、癫痫、颅内出血的有无及其临床随访情况相关联。

材料与方法

对病理科一年内（2016年1月至12月）送检进行细胞学检查的新生儿CSF样本进行回顾性研究。检索CSF细胞计数，并复查细胞涂片的细胞密度、细胞类型、比例和背景情况，并与生化、微生物学及临床放射学检查结果相关联。

结果

共纳入213份样本，其中男性140例，女性73例，年龄范围为0至28天（平均7.3天）。CSF细胞计数平均值为5.48/立方毫米（0至90个细胞/立方毫米）。最常见的细胞学发现是偶见淋巴细胞或无细胞CSF（63.9%）。CSF白细胞计数和蛋白水平与s C反应蛋白呈显著相关性。CSF细胞学检查显示新生儿年龄与血液中性粒细胞百分比之间存在显著相关性（P = 0.0158）。颅内出血病史显示红细胞存在频率显著更高（P = 0.0147）。