Kaji A, Imai H, Maeda T, Watanabe S
Histochemistry. 1986;85(3):203-8. doi: 10.1007/BF00494805.
We developed a new histochemical method based on endogenous oxidase activity for the detection of cutaneous nerves in guinea-pig lips. After the application of a reaction mixture containing 0.14 mM 3,3'-diaminobenzidine(DAB) and 15 mM nickel ammonium sulfate in Tris-HCl buffer (pH 7.6), many blue-black sensory fibers were observed, i.e., dermal nerve fiber bundles, perifollicular plexuses, Merkel-cell endings, and Meissner-like nerve endings. The autonomic fibers of the skin were not stained. Electron microscopy revealed that the reaction product was mainly localized on mitochondrial cristae. The addition of 0.01 M sodium azide completely inhibited the reaction. This technique is simple and preferentially stains sensory nerves.
我们基于内源性氧化酶活性开发了一种新的组织化学方法，用于检测豚鼠唇部的皮肤神经。在含有0.14 mM 3,3'-二氨基联苯胺（DAB）和15 mM硫酸镍铵的Tris-HCl缓冲液（pH 7.6）的反应混合物应用后，观察到许多蓝黑色的感觉纤维，即真皮神经纤维束、毛囊周围丛、默克尔细胞终末和类梅斯纳神经终末。皮肤的自主神经纤维未被染色。电子显微镜显示反应产物主要定位于线粒体嵴上。添加0.01 M叠氮化钠完全抑制了该反应。该技术简单且优先染色感觉神经。
