Crisp Amy R, Short Bryn, Rowan Laurence, Ramage Gordon, Rehman Ihtesham U R, Short Robert D, Williams Craig
Engineering Department, Lancaster University, Bailrigg, Lancaster, LA1 4YW, UK.
School of Medicine, Dentistry and Nursing, MVLS, University of Glasgow, Glasgow, G12 8QQ, UK.
Biofilm. 2023 Jun 30;6:100141. doi: 10.1016/j.bioflm.2023.100141. eCollection 2023 Dec 15.
Diagnosing biofilm infections has remained a constant challenge for the last 50 years. Existing diagnostic methods struggle to identify the biofilm phenotype. Moreover, most methods of biofilm analysis destroy the biofilm making the resultant data interpretation difficult. In this study we introduce Fourier Transform Infra-Red (FTIR) spectroscopy as a label-free, non-destructive approach to monitoring biofilm progression. We have utilised FTIR in a novel application to evaluate the chemical composition of bacterial biofilms without disrupting the biofilm architecture. (RP62A) was grown onto calcium fluoride slides for periods of 30 min-96 h, before semi-drying samples for analysis. We report the discovery of a chemical marker to distinguish between planktonic and biofilm samples. The appearance of new proteins in biofilm samples of varying maturity is exemplified in the spectroscopic data, highlighting the potential of FTIR for identifying the presence and developmental stage of a single biofilm.
在过去的50年里，诊断生物膜感染一直是一项持续存在的挑战。现有的诊断方法难以识别生物膜表型。此外，大多数生物膜分析方法会破坏生物膜，导致难以对所得数据进行解释。在本研究中，我们引入傅里叶变换红外（FTIR）光谱法，作为一种无标记、非破坏性的方法来监测生物膜的进展。我们将FTIR应用于一个新的领域，以评估细菌生物膜的化学成分，同时不破坏生物膜结构。将（RP62A）在氟化钙载玻片上培养30分钟至96小时，然后对样品进行半干燥以进行分析。我们报告发现了一种化学标记物，可区分浮游生物和生物膜样本。光谱数据例证了不同成熟度生物膜样本中新蛋白质的出现，突出了FTIR在识别单个生物膜的存在和发育阶段方面的潜力。
